Results 351 to 360 of about 1,287,734 (381)
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Journal of Pathology, 2019
TP53 mutations are considered a surrogate biomarker of the serous‐like ‘copy number high’ molecular subtype of endometrial carcinoma (EC). In ovarian carcinoma, p53 immunohistochemistry (IHC) accurately reflects mutational status with almost 100 ...
N. Singh +7 more
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TP53 mutations are considered a surrogate biomarker of the serous‐like ‘copy number high’ molecular subtype of endometrial carcinoma (EC). In ovarian carcinoma, p53 immunohistochemistry (IHC) accurately reflects mutational status with almost 100 ...
N. Singh +7 more
semanticscholar +1 more source
Current Protocols in Cytometry, 1995
AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
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AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
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Microwaves for immunohistochemistry
Micron, 1994Microwaves are now widely used in immunohistochemistry for fixing and stabilizing tissue prior to embedding and cutting, for antigen retrieval and for immunoincubations. These techniques can be used for frozen sections and for material embedded in paraffin and plastic. Material prepared in this way shows high contrast in light microscopy. In principle,
L. P. Kok, Mathilde E. Boon
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Current Protocols in Immunology, 2002
AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or from suspension or adherent cultures, or fixed ...
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AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or from suspension or adherent cultures, or fixed ...
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Fluorescent Immunohistochemistry
2021Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry. However, analysis of
Vanessa, Moreno +2 more
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Immunohistochemistry of retinoblastomas
Journal of Neuro-Oncology, 1987Immunohistochemical stains using neuronal and glial marker proteins were applied to retinoblastomas tissues from 14 children. Among the neurofilament triplet proteins, NF68Kd positive cells were observed in 12. Few NF160Kd positive cells were noted in 2, and NF210Kd positive cells were not detected.
Inomata H +4 more
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Immunocytochemistry and Immunohistochemistry
2003Immunohistochemistry allows the specific histochemical localization of many diverse classes of organic substances, including inflammatory markers ranging from biogenic amines to macromolecular cytokines and their receptors. Corresponding ligands and their receptors can be demonstrated simultaneously by double-labeling protocols (1), since the fixation ...
Springer, J, Fischer, A
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Standardization in Immunohistochemistry
Applied Immunohistochemistry & Molecular Morphology, 2001Over the last few years, the role of immunohistochemical analysis has been changing from that of an ancillary diagnostic technique to that of a stand-alone diagnostic method, the results of which determine treatment approach. With this change comes a need for increasing standardization of both preanalytical and analytical methods, so that results ...
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Immunohistochemistry Microarrays
Analytical Chemistry, 2017Immunohistochemistry (IHC) on tissue sections is widely used for quantifying the expression patterns of proteins and is part of the standard of care for cancer diagnosis and prognosis, but is limited to staining a single protein per tissue. Tissue microarray and microfluidics staining methods have emerged as powerful high throughput techniques, but ...
Huiyan Li +5 more
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Enzymatic Immunohistochemistry
2021Immunohistochemistry (IHC) is a powerful laboratory technique that is widely used in a variety of settings, including clinical medicine and research. Enzymatic immunohistochemistry refers to the process of detecting cell surface proteins or antigens using enzyme-labeled antibodies.
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