Synthetic Multidomain Proteins Containing Unstructured and α‑Helical Linkers Reveal a Differential Impact of Molecular Crowding on Catalytic Activity and Conformation. [PDF]
ACS OmegaSultana A +3 more
europepmc +1 more source
Impact of fire frequency on woody community structure and soil nutrients in the Kruger National Park
, 2000 Charlie M. Shackleton, Robert J. Scholes
openalex +2 more sources
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
tRNA modifications as regulators of bacterial virulence and stress responses. [PDF]
PLoS PathogTeixeira C, Vandenesch F, Moreau K.
europepmc +1 more source
HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Organizational resilience in nursing: a concept analysis using Rodgers' evolutionary approach. [PDF]
Health Res Policy SystPeng Y +6 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Impact of artificial intelligence-driven urban renewal strategies green economic efficiency and resident health in China. [PDF]
Front Public HealthPeng J, Fu X, Peng Y, Ding Y.
europepmc +1 more source
Carbonate Melt Fragments in Resurge Deposits from the Lockne Impact Structure, Sweden [PDF]
, 2012 Lee, M. +5 more
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