Results 121 to 130 of about 555,304 (261)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Strengthening antimicrobial resistance policies: lessons from the design and implementation phase of the EU-JAMRAI 2 Sustainability Guidance Tool. [PDF]

Public Health Action
Davido B, Kharkhordine M, Merillon D, Fuentes-Braesch M.   +3 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Clinical Outcomes and Learning Curve of Endoscopic Ultrasound-Guided Hepaticogastrostomy During the Implementation Phase in Inexperienced Centers: A Multicenter Retrospective Study. [PDF]

DEN Open
Kaneko J, Satoh T, Kobayashi Y, Suzuki A, Kawaguchi S.   +4 more
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Assessment of acute stroke care, stroke metrics and patient outcomes: analysis from the pre-implementation phase of the IMPETUS stroke study. [PDF]

Front Neurol
Gupta S, Bhatia R, Srivastava MVP, Haldar P, Singh MB, Salunkhe M, Longkumer I, Prasad D, Sarkar R, Sardana V, Maheshwari D, Bhushan B, Verma A, Dongre N, Sahu N, Panda S, Anand S, Ray BK, Puri I, Zanzmera P, Gamit A, Bhoi SK, Jha M, Samal P, Gopi S, Raju GB, Bhardwaj A, Sibia RS, Kaur R, Tiwari A, Kumar N, Singh M, Bala K, Dabla S, Chawla MPS, Garg J, Chandan S, Malik R, Iype T, Pushpa C, Kumar A, Ranjan A, Garg R, Sulena S, Darole P, Chhina G, Shah S, Shah S, Ranga G, Nath S, Saluja A, Ghotekar LH, Vishnu VY, Rajan R, Gupta A, Vibha D, Singh RK, Pandit AK, Agarwal A, Rohila A, Khera P, Tiwari S, Bhaskar S, Garg M, Dey A, Guru S, Naik S, Kumar TS, Dhar M, Agrawal N, Patel M, Joshi P.   +71 more
europepmc   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Lessons learned from the pre-implementation phase of a patient navigation intervention to increase patient portal enrollment in Federally Qualified Health Centers. [PDF]

Front Health Serv
Matthews AK, Elkefi S, George M, Cassells A, Tobin JN.   +4 more
europepmc   +1 more source