Results 161 to 170 of about 1,057,375 (305)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Implementation strategies for the WHO Safe Childbirth Checklist: a scoping review. [PDF]

BMJ Open
Gama ZADS, Semrau KEA, Rosendo TMSS, Freitas MR, Saraiva COPO, Westgard CM, Mita C, Tuller DE, Freitas KMS, Molina RL.   +9 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Construction and empirical analysis of a five-tier governance framework for unlocking the value of public data

大数据
Addressing the shift in the development and utilization of public data from the question of "whether to open it up" to "how to govern it to realize its value", the dilemma that institutional rules, organizational coordination, resource management and ...
Chen Yishuo, Wang Xiaohan, Zhang Xiaotong, Zhao Zhanbo   +3 more
doaj  

Insights on the implementation strategies for a potential new tuberculosis vaccine in South Africa. [PDF]

PLOS Glob Public Health
Thomas KA, Okoro C, Ntshiqa T, Damovsky V, Goldberg D, Ramokhoase O, Clark RA, Jassat W, Churchyard GJ, Abdullah F, Kubjane M, Seeley J, Charalambous S, White RG.   +13 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Introduction to Market Systems Approaches in Project Implementation, 11–20 August 2020 [PDF]

, 2020
Ultimate Business Strategies, Ltd
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Reporting and Specifying the Implementation Strategies Used in a National Programme of Primary Care Youth Mental Health. [PDF]

Early Interv Psychiatry
Moore J, O'Brien G, Cullinan S, Duffy J.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source