Results 61 to 70 of about 3,160 (217)

Single‐subject designs in character education: Methods for rigorous, contextual, and practitioner‐led research

British Educational Research Journal, EarlyView.
Abstract Character education research is often constrained by blunt methodological tools. Surveys capture breadth without depth; case studies offer richness but lack replicability; and randomised controlled trials (RCTs), though indispensable at the policy level, are costly, disruptive and ill‐suited to everyday practice with individual pupils.
Shane McLoughlin
wiley   +1 more source

Metabolic Flux Analysis Reveals Cell Line‐Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Chinese hamster ovary (CHO) cells are the leading host for recombinant therapeutic protein production in the biopharma industry. In this study, we investigated how feeding acidic forms of tricarboxylic acid (TCA) cycle intermediates—malic acid, succinic acid, and α‐ketoglutaric acid—affects cell culture performance and metabolism in two ...
Sarah A. Sacco, Kevin J. Ruiz‐Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D. Young   +8 more
wiley   +1 more source

Weak Solutions for a p-Laplacian Impulsive Differential Equation

Abstract and Applied Analysis, 2013
By the virtue of variational method and critical point theory, we give some existence results of weak solutions for a p-Laplacian impulsive differential equation with Dirichlet boundary conditions.
Wei Dong, Jiafa Xu, Xiaoyan Zhang
doaj   +1 more source

Comparative Analysis of Bioreactor Design and Scale‐Up for Cultivated Meat Using Monte Carlo‐Based Timescale Modeling

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cultivated meat offers a sustainable alternative to conventional livestock production but faces significant scale‐up challenges, particularly in bioreactor design for mammalian cell suspension cultures. This study presents a comparative analysis of stirred tank reactors (STR), bubble columns (BC), and airlift reactors (ALR) to assess their ...
Pieter Jan Theodoor Brorens, Marcel Ottens, Cees Haringa   +2 more
wiley   +1 more source

High Cell Density Cultivation of HEK293 Cells Using a 2L Membrane‐Stirred Bioreactor

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The use of perfusion systems for the continuous cultivation of animal cells at high cell density (HCD) for the production of recombinant proteins, viruses, and viral vectors has many advantages. Besides an enhancement of volumetric productivity and high cell viability, the product quality can be improved through a steady‐state environment ...
Lennart Jacobtorweihe, Yasemin van Heuvel, Kathrina Scheiermann, Patrick Bongartz, Yvonne Genzel, Udo Reichl   +5 more
wiley   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Ordinary dichotomy and perturbations of the coefficient matrix of the linear impulsive differential equation

International Journal of Mathematics and Mathematical Sciences, 1991
In the present paper it is proved that the ordinary dichotomy is preserved under perturbations of the coefficient matrix of the linear impulsive differential equation.
N. V. Milev, D. D. Bainov
doaj   +1 more source

A new comparison principle and its application to nonlinear impulsive functional integro-differential equations

Advances in Difference Equations, 2018
In this article, we first create a new comparison principle for a nonlinear impulsive boundary problem involving different deviating arguments. Then we employ the new result and iterative method to study the existence of the max-minimal solution of a ...
Yingmin Hou, Lihong Zhang, Guotao Wang
doaj   +1 more source

A General Workflow for Tangential Flow Filtration Perfusion Scale‐Up

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Tangential flow filtration (TFF) perfusion using hollow‐fiber modules offers substantial advantages over other mammalian cell retention technologies. Recently, increased awareness of Starling flow has helped to enhance filtration performance to similar, or even higher levels compared to alternative technologies.
Patrick Romann, Ken Lee, Venkatesh Natarajan, Daniel Ley, Claes Nymand Nilsson, David Garcia‐Munzer, Dominik Schieman, Mahbod Hajighasemi, Alex Zaytsev, Jason Friedman, Loic Chappuis, Tobias Habicher, Colin Jaques, Alex Cohen, Matthew Gagnon, Edward Chan, Daniel J. Karst, Philip Giller, Antony Sibilia, Andrew L. Zydney, Thomas K. Villiger   +20 more
wiley   +1 more source

On the importance of including both sexes in animal studies – insights from home‐cage monitoring

Biological Reviews, EarlyView.
ABSTRACT A review of behavioural studies using home‐cage monitoring (HCM) systems revealed that over 61% of studies used only male subjects, with only 24% including both sexes, despite evidence of substantial behavioural differences between male and female animals. This bias could influence the outcomes of biomedical research.
Maša Čater, Adam Dušek, Aleksandra Bartelik, Silvia Mandillo, Elisabetta Golini, Mara Rigamonti, Rasneer Sonia Bains, Hamish Forrest, Marion Rivalan, Melissa Long, Anna Kiryk, Lars Lewejohann, Nuno Henrique Franco   +12 more
wiley   +1 more source