FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Interleukin-4 Supplementation During In Vitro Culture Modulates the Expression of Stress- and Immune-Related Genes During Porcine Blastocyst Development. [PDF]
Reprod Domest AnimGarcia-Canovas M +7 more
europepmc +1 more source
FEBS Open Bio, EarlyView.KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph +7 more
wiley +1 more source
Application of Nanoparticles in Plant In Vitro Culture for Micropropagation and Secondary Metabolite Production: A Review. [PDF]
Plants (Basel)Semenova NA +5 more
europepmc +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
A Two-Phase System for the In Vitro Culture of <i>Agave guiengola</i> Gentry. [PDF]
Plants (Basel)Maldonado-Zavala I +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Ontogeny of in vitro rooting processes in Eucalyptus globulus
, 2004 Calderon, Ximena. Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile.In the present work, histological changes observed at the base of Eucalyptus globulus shoots in in vitro culture are described.
De Garcia, E. +2 more
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Influence of the In Vitro Culture System on the Preferential Accumulation of Bioactive Compounds in <i>Stevia rebaudiana</i> Shoots. [PDF]
Plants (Basel)Martínez-Oró D +2 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source