Results 171 to 180 of about 272,120 (295)

Establishment of in vitro models for Neutrophil granulocytes

, 2013
Neutrophile Granulozyten bilden die erste Abwehrlinie im angeborenen Immunsystem und reagieren auf eindringende Pathogene und Verletzungen. Um Neutrophile in ?in vitro? zu studieren werden oft HL-60, eine humane Leukämiezellinie, verwendet. Durch Induktion mit Retinolsäure können die Zellen zu Neutrophilen reifen.
openaire   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Establishment of expression and in vitro refolding systems for protein engineering of wheat gluten [PDF]

, 1991
Tatham, A. S., Thompson, S., Shewry, P. R., Pratt, K. A., Bishop, D. H. L., Madgwick, P. J., Colot, V.   +6 more
core  

Babesia microti: Breaking the culture barrier. [PDF]

Trends Parasitol
Vydyam P, Beri D, Lobo CA.
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Establishment and Characterization of a Long-Term Ovarian Cell Line (SBO) from Asian Seabass (<i>Lates calcarifer</i>) Expressing Germline Stem Cell Markers. [PDF]

Int J Mol Sci
Zhang R, Zhan Z, Zhao M, Li Y, Xu H.
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Establishment of Breast Cancer Organoids: A Systematic Review and Meta-Analysis. [PDF]

Int J Breast Cancer
Rocha VM, Katayama MLH, Amorim LO, de Oliveira BD, Lopez RVM, Folgueira MAAK.   +5 more
europepmc   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source