Results 271 to 280 of about 633,020 (302)
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Isolation and in vitro expansion of human colonic stem cells
Nature Medicine, 2011Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression
Peter Jung +2 more
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Optimal protocols of hematopoietic stem cell expansion in vitro
Cell and Tissue Biology, 2013Hematopoietic stem cells (HSC) effectively and continuously renew a full spectrum of blood cell populations. Bone marrow and umbilical cord blood stem cells transplantation (SCT) restore hematopoietsis, when used in various hematological and oncohematological disorders in adults and children.
R I, Dmitrieva, S V, Anisimov
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Neuraminidase enhances in vitro expansion of human erythroid progenitors
European Cytokine Network, 2016In spite of recent key improvements, in vitro mass production of erythrocytes from human stem cells is still limited by difficulties in obtaining sufficient numbers of erythroid progenitors. In fact, such progenitors are as scarce in the bone marrow as in peripheral blood.
Bodivit, Gwellaouen +6 more
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The Preferential Expansion of Functional CD4+ Lymphocyte Populations In Vitro
Journal of Immunotherapy, 1992We describe a simple and inexpensive chemical procedure for the selective expansion of human CD4+ lymphocytes. The method employs L-leucine methyl ester (LME) to deplete monocytes and large granular lymphocytes, as well as to inhibit growth of CD8+ lymphocytes.
R M, Townsend, P, Simon
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In Vitro Expansion of Stem Cells
1999An in vitro cell bioreactor system faithfully re-creating many physiologic aspects of normal hematopoiesis, including stem cell replication, could serve several important purposes in transplantation medicine. These include (a) selectively producing cells for infusion to meet specific needs in the transplantation setting; (b) selectively purging tumor ...
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2009
Ex-vivo development of human insulin-producing cells is considered a promising approach for the generation of an abundant supply of cells for β-cell replacement therapy for diabetes, which is currently limited by the shortage of islet donors. The most obvious cell source for these approaches is the islets themselves.
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Ex-vivo development of human insulin-producing cells is considered a promising approach for the generation of an abundant supply of cells for β-cell replacement therapy for diabetes, which is currently limited by the shortage of islet donors. The most obvious cell source for these approaches is the islets themselves.
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Clinical application of in vitro expansion of cord blood.
Bone marrow transplantation, 1998Expansion of cord blood (CB) haemopoietic cells has been investigated with the aim of reducing cytopenia following transplantation. We investigated the increase in total nucleated cells, colony-forming cells (CFC), CD34+ cells and long-term culture-initiating cells (LTC-IC) by limiting dilution after a 14-day culture of CB CD34+ cells (5 x 10(3)/ml ...
Denning-Kendall, P. A. +5 more
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In vitro culture and expansion of human limbal epithelial cells
Nature Protocols, 2010Limbal stem cells (LSCs) have an important role in the maintenance of the corneal surface epithelium, and autologous cultured limbal epithelial cell transplantations have contributed substantially to the treatment of the visually disabling condition known as LSC deficiency.
Indumathi, Mariappan +8 more
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In vitro ribosomal ribonucleoprotein transport upon nuclear expansion
Biochemistry, 1980The interdependence of nuclear rRNA release and nuclear size is investigated in macronuclei isolated from Tetrahymena. Nuclei are induced to contract and to expand, without any structural disintegration of the nuclear envelope, by final Ca2+/Mg2+ (3:2) concentrations of 5 and 1.5 mM, respectively.
G, Herlan, G, Giese, F, Wunderlich
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In Vitro And in Vivo Expansion of Stem Cell Populations
Vox Sanguinis, 1998AbstractExpansion of hemopoietic stem cells occurs in vivo following transplantation of limited numbers of bone marrow cells or of highly purified stem cells. Stem cell expansion can in principle be achieved in vitro and also be promoted in vivo by growth factor treatment, notably with thrombopoietin.
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