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Pollen Germination and Pollen Tube Growth in Vitro
1992Pollen germination and pollen tube growth are prerequisites for fertilization and seed development. Because of the involvement of the pistillate tissue in nature, physiologic and biochemical investigations on pollen germination and pollen tube growth in vivo are rather difficult. In vitro germination techniques have therefore been used extensively on a
K. R. Shivanna, N. S. Rangaswamy
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A NEW MEDIUM FOR POLLEN GERMINATION IN VITRO
Acta Botanica Neerlandica, 1982SUMMARY Poly-ethylene glycol (PEG)-400 was found to be superior to sucrose as the major component in media for the germination of Petunia hybrida pollen. PEG promotes pollen tube growth considerably more than sucrose. Pollen tubes grown on PEG resemble the in vivo tubes much more than those grown on sucrose.
Zhang Hong-Qi, A. F. Croes
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In vitro germination of black walnut pollen
Canadian Journal of Botany, 1971Black walnut pollen was germinated on liquid and agar-based media containing 20% sucrose, a requirement for germination and normal tube development. Boron (100 ppm boric acid) added to this medium slightly enhanced germination. Germination occurred only in dense (150+ grains/mm2) populations of pollen on the agar medium. This population effect was not
Geraldine C. Hall, Robert E. Farmer Jr.
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In Vitro Analysis of Cotton Pollen Germination
Agronomy Journal, 2004Crops with economically valuable reproductive structures show the greatest negative discrepancy between average and record yields. To alleviate environmental stress–related yield reductions, a better understanding of the relative sensitivities of pollen development, dehiscence, pollen germination, pollen tube growth, fertilization, and subsequent boll ...
John J. Burke +2 more
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1994
The ability to germinate fresh pollen on a “defined” medium offers opportunities for a number of areas of research. Applications, such as agrichemical assessment, gametophyte selection, etc., have been reported by a number of investigators over the last 25 years or more.
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The ability to germinate fresh pollen on a “defined” medium offers opportunities for a number of areas of research. Applications, such as agrichemical assessment, gametophyte selection, etc., have been reported by a number of investigators over the last 25 years or more.
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Germination in vitro of ryegrass pollen grains
Euphytica, 1973Pollen grains of diploid and tetraploid Italian, perennial and hybrid ryegrass (Lolium spp.) germinated on 20% sucrose solution with 10 ppm each of CaCl2, H3BO3 and EDTA. Pollen tubes emerged within 2–3 minutes after sowing and completed full growth within 1 1/2 hours. The tube elongation showed the expected linear growth rate. The tubes of the haploid
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An Improved Cellophane Method for in Vitro Germination of Recalcitrant Pollen
Stain Technology, 1989The cellophane technique of La Cour and Faberge has been improved by the use of a booklet of filter paper. The booklet consists of seven squares of filter paper stapled together; the cellophane on which the pollen is germinated is placed between the two top leaves of the booklet and the whole soaked in a sucrose-based nutrient medium for 15 min.
M P, Alexander, S, Ganeshan
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In Vitro Germination of Ornithogalum Caudatum Pollen
Transactions of the Kansas Academy of Science (1903-), 1962Ornithogalum caudatum Ait. (Fig. 1) is a member of the Liliaceae family, and occasionally grown as a window or greenhouse plant. It originated in South Africa (Bailey 1916). This research attempted to evaluate characteristics of the plant's pollen and its potential value for future studies in pollen physiology.
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Optimising storage and in vitro germination of Eucalyptus pollen
Australian Journal of Botany, 2007The best sucrose solution for maximum in vitro germination of Eucalyptus pollen was investigated in order to evaluate pollen germination rate as an indicator of pollen viability. In vitro germination of both freshly collected and 1-year-old pollen (stored at 4°C) of Eucalyptus grandis, E. smithii, E. nitens, E. dunnii and E. macarthurii was carried out
Tasmien N. Horsley +2 more
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Pollen germination in vitro and pollen tube growth of Scutellaria baicalensis
China Journal of Chinese Materia Medica, 2011OBJECTIVE To study the pollen germination in vitro and pollen tube growth of Scutellaria baicalensis. METHOD Two years-old S. baicalensis that in the experiment field of Shangluo university were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of S ...
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