Results 171 to 180 of about 2,915,776 (318)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Remineralization Effect of a Strontium-Containing Composite: An In Vitro Study. [PDF]

Materials (Basel)
Martínez-Llop A, Sanz JL, Melo M, Folguera S, Llambés G, Ghilotti J.   +5 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

In vitro models for the blood-brain barrier

, 2005
The aim of the present study was to identify a model for the blood-brain barrier based on the use of a continuous cell line, and to investigate the specificity of this model.
Osterberg, T., Stanimirovic, D., Nilsson, J., Terasaki, T., Fenart, Laurence, Öberg, J.-O., Garberg, P., Mabondzo, Aloïse, M., Raub, T. J., Ball, M., Hurst, D. R., Oberg, J., Hurst, R., Raub, T., Cecchelli, Roméo, Fenart, L., Ball, Marguerite, Mabondzo, A., Stanimirovic, Danica, Nilsson, J. E., Borg, N., Cecchelli, R., Lindmark, T.   +22 more
core  

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Multifunctional Oregano-Derived Plasma Polymer Coatings for Wound Healing Applications: An In Vitro Study. [PDF]

Int Wound J
Romo-Rico J, Ninan N, Hayles A, Krishna SM, Vasilev K, Golledge J, Bright R, Jacob M.   +7 more
europepmc   +1 more source

In vitro soil-less (IVS) rooting medium

, 2006
The principle hypothesis of this thesis is that hypoxia, in agar-based media, compromises rooting in vitro. From a practical point of view this is important because most plant tissue culture activities require the material to be successfully acclimatised
Newell, Christopher Jack
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Effect of Implant Surface Decontamination Procedures on Surface Morphology-In Vitro Study. [PDF]

J Funct Biomater
Özay F, Ersanlı S.
europepmc   +1 more source