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A Technique for Studying Sperm Maturation in vitro

Nature, 1972
IN mammals spermatozoa mature while passing through the epididymis. In the rabbit there are virtually no fertile spermatozoa in the caput epididymidis and fertilizing ability is acquired when, the spermatozoa pass through the corpus epididymidis1,2. Spermatozoa retained by ligatures, in the proximal corpus develop a normal fertilizing ability in 24 h ...
M C Orgebin-Crist, P. L. Tichenor
openaire   +3 more sources

Cellular and In Vitro Techniques

1986
This chapter will consider the attempts that have been made to date to make use of cells in culture to measure toxicity. The past decade has seen some major advances in biotechnology. For some years now it has been possible to isolate individual mammalian genes and to transfer these into bacteria, or indeed to other mammalian cells. By the construction
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In Vitro Clotting Technique for Hemodialysis Circuits

ASAIO Journal, 1999
Physiologic clotting of a dialysis circuit was achieved using an in vitro method. The closed loop method used a circuit filled with defibrinated bovine blood. To achieve clotting, a slow infusion (5-15 ml/hr) of ACD bovine plasma was performed. The rate and amount of infusion of the plasma allowed for careful control of the amount of clotting in the ...
James A. Summerton   +2 more
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In Vivo and In Vitro Starch Digestion: Are Current in Vitro Techniques Adequate?

Biomacromolecules, 2010
The time evolution of the size distributions of (fully branched and debranched) starch molecules during in vivo and in vitro digestion was analyzed using size exclusion chromatography (SEC) and compared. In vivo digesta were collected from the small intestine of pigs fed with raw normal maize starch; in vitro digestion was carried out on the same diet ...
Hasjim, Jovin   +3 more
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Techniques for In Vitro and In Vivo Fertilization in the Rat

2009
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill.
Tadao Serikawa   +5 more
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In Vitro Micronucleus Technique to Predict Chemosensitivity

2005
The study of DNA damage at the chromosome level is an essential part of genetic toxicology because chromosomal mutation is an important event in carcinogenesis. The micronucleus assays have emerged as one of the preferred methods for assessing chromosome damage because they enable both chromosome loss and chromosome breakage to be measured reliably ...
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In Vitro Techniques in Teratology

Toxicology and Industrial Health, 1989
George P. Daston, Robert A. D'amato
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[41] Techniques for the study of microtubule assembly in vitro

1982
Publisher Summary This chapter presents the techniques for the study of microtubule assembly in vitro. Microtubule assembly and disassembly in vitro is frequently monitored by turbidity (light scattering) and electron microscopy. Useful information concerning the mechanism of microtubule assembly has also been obtained with a variety of other ...
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SARS-CoV-2 variant evasion of monoclonal antibodies based on in vitro studies

Nature Reviews Microbiology, 2023
, William T Harvey, Joseph Hughes
exaly  

Somatic Embryogenesis and Alternative In Vitro Techniques

2014
Genetic engineering through in vitro manipulation of the cell seems to be an additional tool for overcoming some of the problems of tea breeding. The prerequisite step for such a technology is an efficient in vitro plant regeneration system. Somatic embryogenesis is considered to be the most efficient regeneration system of tea. In the present chapter,
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