Results 201 to 210 of about 436,071 (266)

Restoring a synthetic methylxanthine degradation operon to its native genes. [PDF]

MicroPubl Biol
Nguyen ST, Ike RN, Kamanu KO, Mishler DM.   +3 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Around the <i>ThermoClock</i>: A precision automated temperature control system for Ex Vivo circadian studies. [PDF]

MethodsX
Zhang KK, Locurto D, Belden M, Price M, Kearney CJ, Huber ME.   +5 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The Preclinical Effects and Mechanisms of Biofield Therapy on Pancreatic Cancer Cell Growth and Metastasis. [PDF]

Cancer Med
Yang P, Wei D, Chakraborty S, Nguyen P, Cusimano A, Deng D, Iqbal S, Nelson M, Cui M, Dai J, Gagea M, Wagner R, Li Y, Cohen L.   +13 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Protocol for acute oxidative stress induction in third-instar Drosophila melanogaster larvae. [PDF]

STAR Protoc
Li J, Pan Y, Zhang Y, Lin S, Liu M, Zhu AJ.   +5 more
europepmc   +1 more source

Assessment of Epithelial Barrier Integrity by TEER and FITC-Dextran Permeability Assays. [PDF]

Bio Protoc
Keser Y, Boulant S, Stanifer ML.
europepmc   +1 more source

Recovery and Expansion of Patient-Derived Glioblastoma Cells After Long-term Cryopreservation. [PDF]

Bio Protoc
Khotchawan W, Lorthongpanich C, Kheolamai P, Sathornsumetee S, Issaragrisil S.   +4 more
europepmc   +1 more source

Measuring Environmental Noise for Babies in Special Care Nurseries and Neonatal Intensive Care Units on a Large Scale: Research Methods. [PDF]

Health Sci Rep
Potter L, Gasparini L, Ertan C, Sung V, Wang J, Wake M.   +5 more
europepmc   +1 more source