Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Label-free in-line characterization of immune cell culture using quantitative phase imaging. [PDF]
Serafini CE +18 more
europepmc +1 more source
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylation. [PDF]
Shi L +4 more
europepmc +1 more source
An innovative efficiency of incubator to enhance organization supportive business using machine learning approach. [PDF]
Li X +7 more
europepmc +1 more source
Machine Learning-Assisted Quantification of Organelle Abundance. [PDF]
Long AJ +7 more
europepmc +1 more source
A Quantitative DNA Fiber Assay to Monitor Replication Fork Progression, Protection, and Restart. [PDF]
Bhattacharya D, Nagaraju G.
europepmc +1 more source
Hypernatremia during the first week of life in very preterm infants and neurodevelopmental outcomes at 3 to 4 years of age: a cohort study. [PDF]
Murakami M +6 more
europepmc +1 more source
Artificial incubation in family poultry farming systems in Brazil: technical feasibility and incubation performance. [PDF]
Alves KCA +11 more
europepmc +1 more source

