Results 131 to 140 of about 54,945 (293)

The genomic Make-Up of a Hybrid Species - Analysis of the Invasive Cottus Lineage (Pisces, Teleostei) in the River Rhine system [PDF]

, 2007
In the past years a new invasive lineage of sculpins (Cottus species complex) has been studied that is currently expanding in the Lower River Rhine. Molecular analysis showed that this lineage has originated through hybridization of Cottus perifretum ...
Stemshorn, Kathryn
core  

Natural Variations of ZmRLR1 Mediate the Root Lodging Resistance of Maize by Regulating Root Ascorbate and Auxin Homeostasis

Advanced Science, EarlyView.
Root lodging severely limits crop yield and quality. We reveal that ZmRLR1, a plasma membrane b‐type cytochrome, regulates lodging resistance in maize through dual roles: modulating intracellular redox balance and functioning as a novel component of clathrin‐mediated endocytosis to influence auxin homeostasis. Moreover, natural variations in the ZmRLR1
Wenshuai Lv, Pengshuai Yan, Huan Chen, Guangxian Li, Yiyue Zhao, Yanhui Chen, Wen‐Xue Li, Qingguo Du   +7 more
wiley   +1 more source

Pathogenicity and functional impact of non-frameshifting insertion/deletion variation in the human genome.

PLoS Computational Biology, 2019
Differentiation between phenotypically neutral and disease-causing genetic variation remains an open and relevant problem. Among different types of variation, non-frameshifting insertions and deletions (indels) represent an understudied group with ...
Kymberleigh A Pagel, Danny Antaki, AoJie Lian, Matthew Mort, David N Cooper, Jonathan Sebat, Lilia M Iakoucheva, Sean D Mooney, Predrag Radivojac   +8 more
doaj   +1 more source

CRISPR‐Cas9‐Loaded Theranostic Liposomes for Enhancing Radiosensitization of Prostate Cancer through POLD4 Gene Editing under Real‐Time MRI Monitoring

Advanced Science, EarlyView.
This study identifies POLD4 as a potential prostate cancer radiosensitization target through transcriptome sequencing. By encapsulating POLD4‐targeted CRISPR/Cas9 plasmids and USPIONs in cationic liposomes, an MRI‐monitored gene‐editing platform is established.
Xuhui Fan, Ruru Zhang, Linjun Yang, Shixiong Chen, Meijuan He, Yongqiang Wang, Linjie Huang, Jianfeng Zeng, Shuwang Wu, Mingyuan Gao, Han Wang   +10 more
wiley   +1 more source

Identification of presumed pathogenic KRT3 and KRT12 gene mutations associated with Meesmann corneal dystrophy. [PDF]

, 2015
PurposeTo report potentially pathogenic mutations in the keratin 3 (KRT3) and keratin 12 (KRT12) genes in two individuals with clinically diagnosed Meesmann corneal dystrophy (MECD).MethodsSlit-lamp examination was performed on the probands and available
Aldave, Anthony J, Chen, Judy L, Chung, Duk-Won D, Deng, Sophie X, Frausto, Ricardo F, Gee, Jessica A, Gee, Katherine M, Lin, Benjamin R   +7 more
core   +1 more source

Systematically Engineering for Efficient Production of 3‐Methyl‐1‐Butanol in Escherichia coli

Advanced Science, EarlyView.
An integrated metabolic engineering strategy was established for high‐level 3‐methyl‐1‐butanol biosynthesis in Escherichia coli. Molecular dynamics‐guided semi‐rational engineering of dihydroxyacid dehydratase uncovered and relieved key catalytic bottlenecks, while adaptive laboratory evolution enhanced strain robustness.
Nanfei Geng, Hao Liu, Haolin Han, Meng Meng, Shaojie Wang, Tianwei Tan, Haijia Su   +6 more
wiley   +1 more source

Novel indel mutation in the N gene of SARS-CoV-2 clinical samples that were diagnosed positive in a commercial RT-PCR assay. [PDF]

Virus Res, 2021
Lee S, Won D, Kim CK, Ahn J, Lee Y, Na H, Kim YT, Lee MK, Choi JR, Lim HS, Lee KR.   +10 more
europepmc   +1 more source

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Advanced Science, EarlyView.
Most rare diseases are caused by genetic mutations. Prime editing (PE) has emerged as a versatile tool capable of inducing diverse mutations without generating DNA double‐strand breaks. Despite its significant clinical potential, PE faces limitations in terms of efficiency and scalability.
Jihyeon Yu, Ju‐Chan Park, Heesoo Uhm, Yong‐Woo Kim, Hyeon Woo Im, Sangsu Bae   +5 more
wiley   +1 more source

Efficient identification of point-mutant monoclonal cells through array tagged high-throughput sequencing [PDF]

Jichu yixue yu linchuang, 2020
Objective To establish an array tagged high-throughput sequencing and pre-polyclonal screening strategy for fast identification of genome-edited cell clones.
HAN Ling, YANG Ke, XUE Zheng, LYU Xiang
doaj  

A deep learning framework for building INDEL mutation rate maps

Abstract Germline short insertions and deletions (INDELs) are pervasive genetic variants that shape genome evolution and contribute to human disease. However, accurately quantifying fine-scale INDEL mutation rates remains challenging due to data limitations and the diversity of INDEL subtypes.
Shuyi Deng, Hui Song, Cai Li
openaire   +1 more source