Results 51 to 60 of about 54,945 (293)
Functional rescue of dystrophin deficiency in mice caused by frameshift mutations using Campylobacter jejuni Cas9 [PDF]
, 2018 Duchenne muscular dystrophy (DMD) is a fatal, X-linked muscle wasting disease caused by mutations in the DMD gene. In 51% of DMD cases, a reading frame is disrupted because of deletion of several exons.
Cappellari, O +9 more
core +2 more sources
An indelible lineage marker for Xenopus using a mutated green fluorescent protein [PDF]
Development, 1996 ABSTRACT We describe the use of a DNA construct (named GFP.RN3) encoding green fluorescent protein as a lineage marker for Xenopus embryos. This offers the following advantages over other lineage markers so far used in Xenopus. When injected as synthetic mRNA, its protein emits intense fluorescence in living embryos. It is non-toxic, and
M, Zernicka-Goetz +6 more
openaire +2 more sources
Novel 61-bp Indel of RIN2 Is Associated With Fat and Hatching Weight Traits in Chickens
Frontiers in Genetics, 2021 The Ras and Rab interactor 2 (RIN2) gene, which encodes RAS and Rab interacting protein 2, can interact with GTP-bound Rab5 and participate in early endocytosis.
Wujian Lin +15 more
doaj +1 more source
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Tumor mutational and indel burden: a systematic pan-cancer evaluation as prognostic biomarkers [PDF]
Annals of Translational Medicine, 2019 Tumor mutational burden (TMB) has been widely studied as a predictive biomarker of response to immune checkpoint inhibitors (ICIs). Besides, evidence suggests frameshift indels are a highly immunogenic mutational class and thus a potentially superior biomarker. However, the general prognostic impact of TMB and indel burden in patients with solid tumors
Hao-Xiang, Wu +11 more
openaire +2 more sources
Indel mutations of sheep PLAG1 gene and their associations with growth traits
Animal Biotechnology, 2021 Pleiomorphic adenoma gene 1 (PLAG1) is mainly expressed in embryonic development, and it is reported to take an effect on the growth performance of mice, cattle, pigs, and sheep. To explore how conservative the PLAG1 is in different sheep breeds, the effects of the two indel variants on the growth traits of the Chinese Luxi blackhead (LXBH) sheep were ...
Yun Pan +7 more
openaire +2 more sources
Genome sequencing of evolved aspergilli populations reveals robust genomes, transversions in A. flavus, and sexual aberrancy in non-homologous end-joining mutants [PDF]
, 2019 BACKGROUND: Aspergillus spp. comprises a very diverse group of lower eukaryotes with a high relevance for industrial applications and clinical implications. These multinucleate species are often cultured for many generations in the laboratory, which can
Amirebrahimi, Mojgan +8 more
core +1 more source
Frontiers in Plant Science, 2017 CRISPR-Cas9 system rapidly became an indispensable tool in plant biology to perform targeted mutagenesis. A CRISPR-Cas9-mediated double strand break followed by non-homologous end joining (NHEJ) repair most frequently results in a single base pair ...
Cynthia J. Denbow +6 more
doaj +1 more source
Frontiers in Genetics, 2023 Background: Genetic testing is becoming more and more accepted in the auxiliary diagnosis and treatment of tumors. Due to the different performance of the existing bioinformatics software and the different analysis results, the needs of clinical ...
Yunlong Cui +7 more
doaj +1 more source
RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes [PDF]
Nucleic Acids Research, 2015 DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene.
Wons, Ewa +2 more
openaire +2 more sources