Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Development and implementation of a finite element program for static electromagnetic field problems
Bibliography: leaves 102-106.A program is presented for the solution of static electromagnetic fields in bounded and unbounded 2- dimensional domains. The program comprises of a mesh generator which descretises the domain into triangular finite elements ...
Gopal, G
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Three-dimensional dynamic response of infinite plate resting on multilayered orthotropic foundation under moving loads. [PDF]
Zhang CL, Cao M, Hong L, Qi YF.
europepmc +1 more source
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Dual-chirality flexagon linkages with infinite eversion and surface reconfigurability. [PDF]
Lin J +6 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Numerical experiments with a continuous L-2-exponential merit function for semi-infinite programming
Here, we present some numerical experiments with a reduction method for solving nonlinear semi-infinite programming (SIP) problems. The method relies on a line search technique to ensure a sufficient decrease of a L_2-exponential merit function.
Pereira, Ana I., Fernandes, Edite M.G.P.
core
Evaluating BOLD functional MRI biophysical simulation approaches: Impact of vascular geometry, magnetic field calculations, and water diffusion models. [PDF]
Berman AJL +4 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Twelve-Month Outcomes of Standalone iStent Infinite in Primary Open-Angle Glaucoma. [PDF]
Yadgarov A, Hornbeak DM, Davidova D.
europepmc +1 more source

