Results 201 to 210 of about 1,320,225 (265)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

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Information technology

Nursing Management, 2008
The RCN has published a guide to help assess the safety and effectiveness of systems such as electronic records that are based on information technology (IT), and that have been or will be introduced in areas of clinical practice.
openaire   +4 more sources

Changing information technology and information technology management

Information & Management, 1997
Abstract Information technology is changing at a rapid rate. A theory has hypothesized that change in IT causes problems for IT managers and that they respond with coping mechanisms to handle these problems. Structured interviews with 16 IT professionals at different organizations identified 31 unique projects that used 86 new ITs which caused 142 ...
John Benamati, Albert L. Lederer, Meenu Singh   +2 more
openaire   +1 more source

Information Technology

Science, 1983
The AAAS Annual Meeting will be held in Detroit on 26-31 May 1983. The inclusive dates were given incorrectly as 21-31 May in the heading of the Preliminary Program (25 Feb., p. 948). The large body of water shown to the south of Detroit on the cover of the issue of 25 February is Lake Erie. The cover legend stated incorrectly that it was Lake Huron.
openaire   +2 more sources

Formated technology and informated action: The nature of information technology

International Journal of Information Management, 2009
This paper is part of a series examining the fundamental nature of informatics: a term used as a convenient umbrella term to stand for the overlapping disciplinary areas of information systems, information management and information technology. The aim of the current paper is to consider some of the universal features of information technology. This is
openaire   +1 more source