Results 51 to 60 of about 2,838,123 (325)

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Information transmission in oscillatory neural activity

, 2008
Periodic neural activity not locked to the stimulus or to motor responses is usually ignored. Here, we present new tools for modeling and quantifying the information transmission based on periodic neural activity that occurs with quasi-random phase ...
A Borst, A Gelperin, AL Hodgkin, B Hutcheon, BJ Richmond, CM Gray, DH Perkel, E Ahissar, ED Adrian, EN Brown, F Pérez, F Rieke, Friedrich T. Sommer, G Laurent, GT Kenyon, H Ishikane, HB Barlow, HC Tuckwell, J Munemori, J OKeefe, JM Fellous, JM Samonds, Kilian Koepsell, LG Nowak, M Berman, M Castelo-Branco, M Laufer, M Sahani, M Stopfer, M Szwed, MA Montemurro, MR Jarvis, N Brenner, NY Masse, P Barrett, P Fries, P Tiesinga, R Barbieri, R Eckhorn, RC Muresan, RM Bruno, RW Rodieck, S Koyama, S Neuenschwander, SW Kuffler, T Ogawa, TE Oliphant, WD Heiss, WJ Freeman, WM Usrey, X Wang, ZN Aldworth   +51 more
core   +1 more source

Trans2express – de novo transcriptome assembly pipeline optimized for gene expression analysis

Plant Methods
Background As genomes of many eukaryotic species, especially plants, are large and complex, their de novo sequencing and assembly is still a difficult task despite progress in sequencing technologies.
Aleksandra M. Kasianova, Aleksey A. Penin, Mikhail I. Schelkunov, Artem S. Kasianov, Maria D. Logacheva, Anna V. Klepikova   +5 more
doaj   +1 more source

Applied aspects of modern non-blind image deconvolution methods

Компьютерная оптика
The focus of this paper is the study of modern non-blind image deconvolution methods and their application to practical tasks. The aim of the study is to determine the current state-of-the-art in non-blind image deconvolution and to identify the ...
O.B. Chaganova, A.S. Grigoryev, D.P. Nikolaev, I.P. Nikolaev   +3 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Loss of proton‐sensing GPR4 reduces tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
G protein‐coupled receptor 4 (GPR4) is a pH‐sensing receptor activated by acidic pH. GPR4 expression is increased in patients with inflammatory bowel disease who are at high risk of developing colorectal cancer. In mouse models, loss of GPR4 attenuated tumor progression. This correlated with increased IL2 and natural killer cell activity.
Leonie Perren, Moana Busch, Pedro A. Ruiz, Ermanno Malagola, Valeria Baumeler, Federica Foti, Adelina Gross, Tobias Grütter, Hendrik Edel, Cordelia Schuler, Kristina Handler, Glenn De Lange, Isabelle C. Arnold, Cheryl de Vallière, Klaus Seuwen, Martin Hausmann, Gerhard Rogler   +16 more
wiley   +1 more source

Origin and diversity of Capsella bursa-pastoris from the genomic point of view

BMC Biology
Background Capsella bursa-pastoris, a cosmopolitan weed of hybrid origin, is an emerging model object for the study of early consequences of polyploidy, being a fast growing annual and a close relative of Arabidopsis thaliana.
Aleksey A. Penin, Artem S. Kasianov, Anna V. Klepikova, Denis O. Omelchenko, Maksim S. Makarenko, Maria D. Logacheva   +5 more
doaj   +1 more source

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, EarlyView.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

Power Allocation for Downlink NOMA Heterogeneous Networks

IEEE Access, 2018
Non-orthogonal multiple access (NOMA) and heterogeneous network (HetNet) are two significant and promising enabling techniques to further improve overall system performance for nextgeneration mobile communication systems.
Dadong Ni, Li Hao, Quang Thanh Tran, Xiaomin Qian   +3 more
doaj   +1 more source

Understanding and measuring mechanical signals in the tumor stroma

FEBS Open Bio, EarlyView.
This review discusses cancer‐associated fibroblast subtypes and their functions, particularly in relation to extracellular matrix production, as well as the development of 3D models to study tumor stroma mechanics in vitro. Several quantitative techniques to measure tissue mechanical properties are also described, to emphasize the diagnostic and ...
Fàtima de la Jara Ortiz, Chiara Cimmino, Maurizio Ventre, Alessandra Cambi   +3 more
wiley   +1 more source