Results 81 to 90 of about 156,213 (211)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Ectomycorrhizal inoculation with Pisolithus tinctorius reduces stress induced by drought in cork oak

, 2018
We investigated whether the performance of cork oak under drought could be improved by colonization with the ectomycorrhizal fungus Pisolithus tinctorius.
Mónica Sebastiana, Rui Malhó, Ana Rita Matos, Malhó, R., Anabela Bernardes da Silva, Sebastiana, M., Alcantara, A., Silvestre, S., André Alcântara, Susana Silvestre, Bernardes da Silva, A, Silvestre, Susana   +11 more
core   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus [PDF]

, 2017
Advanced animal models, such as minipigs, are needed for the development of a globally requested human Chlamydia vaccine. Previous studies have shown that vaginal inoculation of sexually mature Göttingen minipigs with Chlamydia trachomatis resulted in an
Emma Lorenzen, Andersen, Peter, Jan O. Secher, Mette S. Hansen, Frank Follmann, Olsen, Anja W., Hansen, Mette S., Secher, Jan O, Jungersen, Gregers, Goericke-Pesch, Sandra, Hansen, Mette Sif, Anja W. Olsen, Weinreich Olsen, Anja, Hannah Zakariassen, Sandra Goericke-Pesch, Jørgen S. Agerholm, Peter Andersen, Secher, Jan O., Agerholm, Jørgen S., Gregers Jungersen, Follmann, Frank, Zakariassen, Hannah, Lorenzen, Emma, Goericke-Pesch, Sandra Kathrin   +23 more
core   +1 more source

Supplementary Information from Technique-based inoculation against real-world misinformation

, 2022
In recent years, numerous psychological interventions have been developed to reduce susceptibility to misinformation. Inoculation theory has become an increasingly common framework for reducing susceptibility to both individual examples of misinformation
Jon Roozenbeek (9502224), Cecilie S. Traberg (12500183), Sander van der Linden (2142253)   +2 more
core   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Über conjunctivitis gonorrhoica ohne inoculation

, 1884
ÜBER CONJUNCTIVITIS GONORRHOICA OHNE INOCULATION Franciscus Cornelius Donders Library (-) Über conjunctivitis gonorrhoica ohne inoculation (14) (-
Haltenhoff, G.
core  

Inoculation, co-inoculation, and re-inoculation with Rhizobium tropici and Azospirillum brasilense: effects on nodulation, growth, and agronomic performance of common bean

The use of inoculants to meet nitrogen demand in common bean crops is still limited among farmers. However, strategies such as co-inoculation and top-dressing re-inoculation have emerged as promising alternatives capable of improving
Nathan Mickael de Bessa Cunha, Ednaldo Cândido Rocha, Itamar Rosa Teixeira, Alexandre Marcos Sbroggio Filho, Tamires Ester Peixoto Bravo, Antonio Alannilson Neres de Oliveira, Gisele Carneiro da Silva Teixeira, Andressa Laís Caldeira de Souza   +7 more
core   +1 more source