Results 91 to 100 of about 5,302,590 (305)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Impact of Coal Export Decline on East Kalimantan's Mining and Coal Industry

JDE (Journal of Developing Economies)
Two of Indonesia’s top export commodities are coal and lignite. However, there is a decrease in the value of coal exports in 2023. The province in Indonesia with the biggest coal reserves is East Kalimantan.
Abigail Brenda Pasorong Randa, Erwin Agung Nur Rohmat, Lisda Oktaviana, Muhammad Hafiz Albab, Fitri Kartiasih   +4 more
doaj   +1 more source

The table of digital economy policy multi-input-output.

The table of digital economy policy multi-input-output.
Guo Li (174860), Xiaoyi Fu (1947208), Shuai Hong (10512136), Tianzun Wang (17972826)   +3 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Macroeconomia dos estados e matriz interestadual de insumo-produto

Economia Aplicada, 2002
This paper presents the methodology and the results ofthe estimation of a system of state accounts for Brazil. Using the available information on GDP, government revenues and expenditures, international and interstate trade for the states, estimates for
Eduardo A. Haddad, Carlos R. Azzoni, Edson P. Domingues, Fernando S. Perobelli   +3 more
doaj  

Public goods and input-output analysis [PDF]

input output analysis;public goods ...
Ruys, P.H.M.
core  

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Cost Reduction Effects of "pseudo FTAs" in Asia -Application of a price model based on a multilateral I/O table- [PDF]

This paper examines the repercussion effects on the production cost of industries in Asian countries when some countries eliminate tariffs and import commodity taxes on all imports.
Tamamura, Chiharu
core