Results 121 to 130 of about 4,558,127 (287)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Native Son. Pressbook insert.

, 2006
Pressbook Insert, Native Sonhttps://egrove.olemiss.edu/ms_matinee/1115/thumbnail ...
Classic Pictures
core   +1 more source

Biomechanical analysis of femoral component malalignment in medial unicompartmental knee arthroplasty

Scientific Reports
Knee osteoarthritis (KOA) has a high prevalence among the elderly, severely affecting their quality of life and imposing a considerable burden on social health insurance.
Fangxing Wang, Jie Zhao, Weijie He, Huazheng He, Qiang Wang   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Effect of design and surgical parameters variations in mobile‐bearing versus fixed‐bearing unicompartmental knee arthroplasty: A finite element analysis

Journal of Experimental Orthopaedics
Purpose Unicompartmental knee arthroplasty (UKAs) are available in the market as fixed‐ and mobile‐bearing (FB and MB) and can be characterised by a different set of design parameters in terms of geometries, materials and surgical approaches, with ...
Thomas Luyckx, Edoardo Bori, Rachele Saldari, Sara Fiore, Virginia Altamore, Bernardo Innocenti   +5 more
doaj   +1 more source

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Sapuridis, Effie
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Deconstructing the steps of pull-type PEG tube insertion

VideoGIE
Video 1Deconstructed steps of the pull-type PEG insertion technique.
Ravishankar Asokkumar, Carlos Paolo D. Francisco, Lim Kim Wei, Rajesh Ravi, Mark Cheah, Roy Soetikno   +5 more
openaire   +3 more sources

Construction of miniature turning tools with insert

, 2016
The main aim of this bachelor thesis is to design a set of miniature turning tools with insert. Elaboration is divided into four chapters. In the first chapter we can find research, which deals with the issue of the construction of turning tools.
Balhar, David
core