Results 1 to 10 of about 3,988 (161)

The GET insertase exhibits conformational plasticity and induces membrane thinning [PDF]

Nature Communications, 2023
The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum.
Melanie A. McDowell, Michael Heimes, Giray Enkavi, Ákos Farkas, Daniel Saar, Klemens Wild, Blanche Schwappach, Ilpo Vattulainen, Irmgard Sinning   +8 more
doaj   +3 more sources

SND3 is the membrane insertase within a distinct SEC61 translocon complex [PDF]

Nature Communications
During the biogenesis of most eukaryotic integral membrane proteins (IMPs), transmembrane domains are inserted into the endoplasmic reticulum membrane by a dedicated insertase or the SEC61 translocon.
Tzu-Jing Yang, Saumyak Mukherjee, Julian D. Langer, Gerhard Hummer, Melanie A. McDowell   +4 more
doaj   +3 more sources

Convergent evolution links molybdenum insertase domains with organism-specific sequences [PDF]

Communications Biology
In all domains of life, the biosynthesis of the pterin-based Molybdenum cofactor (Moco) is crucial. Molybdenum (Mo) becomes biologically active by integrating into a unique pyranopterin scaffold, forming Moco. The final two steps of Moco biosynthesis are
Miriam Rabenow, Eduard Haar, Katharina Schmidt, Robert Hänsch, Ralf R. Mendel, Kevin D. Oliphant   +5 more
doaj   +3 more sources

Architecture and conformational dynamics of the BAM-SurA holo insertase complex. [PDF]

Sci Adv
The proper folding of outer membrane proteins in Gram-negative bacteria relies on their delivery to the β-barrel assembly machinery (BAM) complex. The mechanism by which survival protein A (SurA), the major periplasmic chaperone, facilitates this process
Lehner PA, Degen M, Jakob RP, Modaresi SM, Callon M, Burmann BM, Maier T, Hiller S.   +7 more
europepmc   +2 more sources

The structural principles underlying molybdenum insertase complex assembly. [PDF]

Protein Sci, 2023
Within the cell, the trace element molybdenum (Mo) is only biologically active when complexed either within the nitrogenase‐specific FeMo cofactor or within the molybdenum cofactor (Moco).
Hassan AH, Ihling C, Iacobucci C, Kastritis PL, Sinz A, Kruse T.   +5 more
europepmc   +2 more sources

The ER membrane complex (EMC) can functionally replace the Oxa1 insertase in mitochondria.

PLoS Biology, 2022
Two multisubunit protein complexes for membrane protein insertion were recently identified in the endoplasmic reticulum (ER): the guided entry of tail anchor proteins (GET) complex and ER membrane complex (EMC).
Büsra Güngör, Tamara Flohr, Sriram G Garg, Johannes M Herrmann   +3 more
doaj   +3 more sources

MTCH2 is a mitochondrial outer membrane protein insertase. [PDF]

Science, 2022
In the mitochondrial outer membrane, tail-anchored (TA) proteins play critical roles in cytoplasmic-mitochondrial communication. Using genome-wide CRISPRi screens, we identify factors involved in mitochondrial TA biogenesis in human cells.
Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, Weissman JS, Voorhees RM.   +13 more
europepmc   +2 more sources

Monitoring the binding and insertion of a single transmembrane protein by an insertase

Nature Communications, 2021
The insertion and folding nascent or fully synthesized polypeptides into membranes is assisted by insertases. Here, the authors use a range of biophysical approaches to provide molecular details of how the transmembrane insertase YidC facilitates the ...
Pawel R. Laskowski, Kristyna Pluhackova, Maximilian Haase, Brian M. Lang, Gisela Nagler, Andreas Kuhn, Daniel J. Müller   +6 more
doaj   +2 more sources

Function of Molybdenum Insertases [PDF]

Molecules, 2022
For most organisms molybdenum is essential for life as it is found in the active site of various vitally important molybdenum dependent enzymes (Mo-enzymes).
Tobias Kruse
doaj   +2 more sources

The mammalian protein MTCH1 can function as an insertase. [PDF]

J Cell Sci
The outer mitochondrial membrane (OMM) hosts a variety of proteins such as import machineries, enzymes, fission/fusion factors, and pore proteins. In Saccharomyces cerevisiae, the MIM complex, consisting of Mim1 and Mim2, mediates the insertion of α ...
Dimogkioka AR, Elias A, Rapaport D.
europepmc   +2 more sources