Results 91 to 100 of about 648,594 (289)

Solving Institutional Collective Action Problems in Multiplex Networks

, 2014
This essay extends theoretical arguments pertaining to single (uniplex) networks on how to solve coordination and cooperation problems associated with institutional collective action to multiplex networks constituting both formal and informal ...
Berardo, Ramiro, Shrestha, Manoj, Feiock, Richard   +2 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Approach to the Organisational Complexity in Terms of Network and Intellectual Capital Concepts [PDF]

The viability of the systems depends on the way of adaptation of the internal complexity to the environmental complexity. Under structural aspect, any complex system represents a network. Complexity may be estimated on the basis of density and of the non-
Vadim DUMITRASCU, Roxana Arabela DUMITRASCU   +1 more
core  

Searchers' assessments of task complexity for web searching

, 2004
The complexity of search tasks has been shown to be an important factor in searchers' ability to find relevant information and their satisfaction with the performance of search engines.
Bell, David J., David J. Bell, Ruthven, Ian, Ian Ruthven   +3 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Working with institutional complexity: a study of cross-sector partnerships

, 2019
In today's dynamic world, organizations have to deal with incompatible institutional demands that arise as the result of different institutional logics and work within such institutional complexity so they can survive and function.
Karakulak, Ozgu
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

From the Habit of Control to Institutional Enablement: Re-envisioning the Governance of Social-Ecological Systems from the Perspective of Complexity Sciences [PDF]

, 2014
Due to the inherent uncertainty in predicting the evolution of phase-spaces in social-ecological systems (SESs), these systems cannot be “optimally” managed through top-down, command and control type of governance designs.
Arne Bomblies, Brian Beckage, Zia, Asim, Koliba, Christopher, Kauffman, Stuart, Stuart Kauffman, Gabor Vattay, Bomblies, Arne, Asim Zia, Christopher Koliba, Beckage, Brian, Vattay, Gabor   +11 more
core   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source