Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Includes bibliographical references (p. ).The purpose of this study was to examine the effectiveness of a model of instruction for English language learners that may serve as an alternative to the Sheltered Instruction Observation Protocol model.
Hancock, Leslie A.
core
HazAPP: Study protocol of a randomized controlled trial to enhance parental instruction of teens' hazard anticipation skill during the learner phase of licensure. [PDF]
O'Neal EE +3 more
europepmc +1 more source
Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara +10 more
wiley +1 more source
In today's globalised educational landscape, the relationship between EnglishMedium Instruction (EMI) and intercultural competence (IC) in higher education is paramount. As institutions increasingly adopt EMI, understanding its interconnectedness with IC
Kremer , Marcelo, Pinto , Susana
core +1 more source
Dictogloss-based instruction and conjunction use in Palestinian tenth-grade EFL learners. [PDF]
Abukhiran AJ, Ozuturk G.
europepmc +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Structured Digital Device Instruction for Older Adults With Stroke During Convalescent Rehabilitation: Prospective Feasibility Study. [PDF]
Ito D +12 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Immersive virtual reality vs. traditional instruction for short-term learning and follow-up outcomes in the Bobath concept: a randomized controlled trial. [PDF]
Cinarli S +4 more
europepmc +1 more source

