Results 51 to 60 of about 1,949,085 (259)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Pre-validation methods for developing a patient reported outcome instrument [PDF]

, 2011
Background: Measures that reflect patients' assessment of their health are of increasing importance as outcome measures in randomised controlled trials.
Ramsay, Craig R, Burr, JM, Hamzah, J. C., Burr Jennifer M, Susan E Campbell, Francis, JJ, Prior, M., Che Hamzah, Jemaima, Azuara-Blanco, Augusto; id_orcid, Campbell, S. E., Castillo, MM, Prior Maria E, Maria E Prior, Castillo, Mayret M, Hamzah, Jemaima Che, Francis, Jillian J., Ramsay, CR, Prior, Maria E, Hamzah, JC, Campbell Susan E, Campbell, SE, Francis, Jillian J, Jemaima Che Hamzah, Prior, Maria E., Azuara-Blanco, A., Mayret M Castillo, Ramsay, Craig R., Ramsay, C. R., Castillo Mayret M, Francis, J., Castillo, M, Castillo, Mayret M., Burr, J. M., Prior, ME, Campbell, Susan E, Jillian J Francis, Craig R Ramsay, Burr, Jennifer M., Jennifer M Burr, Azuara-Blanco, A, Azuara-Blanco, Augusto, Burr, Jennifer M, Campbell, Susan E., Francis Jillian J, Hamzah Jemaima, Ramsay Craig R, Augusto Azuara-Blanco, Azuara-Blanco Augusto   +47 more
core   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

The analogue computer as a scientific instrument [PDF]

The users of analogue computing employed techniques that have important similarities to the ways scientific instruments have been used historically. Analogue computing was for many years an alternative to digital computing, and historians often frame the
Care, Charles
core  

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

TerraSAR-X Instrument Operations Rooted in the System Engineering and Calibration Project

, 2010
This paper presents the TerraSAR-X instrument operations embedded into the Instrument Operations and Calibration Segment. Special focus is on the data-take (DT) command generation.
Steinbrecher, Ulrich, Schulze, Daniel, Ulrich Steinbrecher, Mittermayer, Josef, Johannes Böer, Josef Mittermayer, Daniel Schulze, Böer, Johannes   +7 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Instrument-based Tests for Measuring Anterior Chamber Cells in Uveitis: A Systematic Review

, 2019
Purpose: New instrument-based techniques for anterior chamber (AC) cell counting can offer automation and objectivity above clinician assessment. This review aims to identify such instruments and its correlation with clinician estimates.
Livia Faes (779621), Sophie Beese (7255025), Matthew E. Round (7255031), Ameenat L. Solebo (7255022), Jesse Panthagani (7255034), Pearse A. Keane (7255046), Tasanee Braithwaite (7255028), Aditya U. Kale (7255037), David J. Moore (250061), Xiaoxuan Liu (167790), Alastair K. Denniston (5697558), Didar Abdulla (7255043), Thomas W. McNally (7255040)   +12 more
core   +1 more source

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source