Results 191 to 200 of about 1,032,894 (314)

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

<i>Trans</i>-Chalcone Reduces Inflammation and Pain Triggered by Superoxide Anion: Neuronal and Non-Neuronal Mechanisms. [PDF]

J Inflamm Res
Piva M, Manchope MF, Barbosa-Costa F, Bianchini BHS, Andrade KC, Silva LC, Calixto-Campos C, Rasquel-Oliveira FS, Fattori V, Zarpelon-Schutz AC, Camilios-Neto D, Borghi SM, Casagrande R, Verri WA.   +13 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Multidimensional Digital Approach to Pain Assessment in Sickle Cell Disease: A Feasibility Study in Ghana. [PDF]

J Pain Res
Obasohan ME, Asare EV, Jonassaint C, Owusu-Ansah AT, Segbefia C, Mamauag E, Dei-Adomakoh Y, Hillery CA, Oluwole O.   +8 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Endothelial-erythrocyte glycocalyx exchange enables liquid biopsies of endothelial function. [PDF]

Nat Commun
Butler MJ, Ramnath RR, Crompton M, Aldam J, Gamez M, Down C, Heffer C, Neal C, Li J, Qiu Y, Carey L, Skinner L, Cross S, Yu Y, Sutak J, Bills V, Welsh GI, Foster RR, Satchell SC.   +18 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Walking dose associated with lower cancer-related fatigue among patients with breast cancer. [PDF]

J Natl Cancer Inst
Mattick LJ, Li CS, Lin PJ, Peppone LJ, Magnuson A, Melnik M, Gross H, Onitilo AA, Kleckner AS, Mustian KM, Janelsins MC.   +10 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

High-Frequency Global Postoperative Status PROMs Track Pain Peaks and Analgesic Use After Degenerative Lumbar Spine Surgery. [PDF]

Global Spine J
Lenga P, Fleige R, Blumenstock MC, Ganzinger M, Ille S, Krieg SM, Dugas M.   +6 more
europepmc   +1 more source