Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
A comparison of knowledge, attitude and intention to use donor human milk among mothers of preterm and term infants in Jos, Nigeria. [PDF]
Diala UM +5 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Predictors of the intention to use integrative medicine in psychiatric hospitals. [PDF]
Schaub C +7 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Actual use, intention to use, and user attitudes toward mobile health applications for coronary heart disease self-management: a systematic review and meta-analysis. [PDF]
Birhanu TE, Vlahu-Gjorgievska E, Win KT.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Intention to use smartphone medical applications among patients with chronic disease in southwest Ethiopia: an extended UTAUT2 model approach. [PDF]
Butta FW +11 more
europepmc +1 more source
Understanding Nurses' Intention to Use Artificial Intelligence Technologies in Their Clinical Practice: A Survey-Based Configurational Analysis. [PDF]
Raymond L, Paré G, Doyon O, Wagner G.
europepmc +1 more source
Determinants of contraceptive use and intention to use among youth 15-24 years from a remote pastoral community in Northeastern, Uganda. [PDF]
Ojanduru L +3 more
europepmc +1 more source

