Demystifying speckle field interference microscopy [PDF]
Scientific Reports, 2022 Dynamic speckle illumination (DSI) has recently attracted strong attention in the field of biomedical imaging as it pushes the limits of interference microscopy (IM) in terms of phase sensitivity, and spatial and temporal resolution compared to ...
Azeem Ahmad +2 more
doaj +6 more sources
Computational interference microscopy enabled by deep learning [PDF]
APL Photonics, 2021 Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method due to its partially coherent illumination and common path interferometry geometry.
Yuheng Jiao +5 more
doaj +5 more sources
Gradient light interference microscopy for 3D imaging of unlabeled specimens [PDF]
Nature Communications, 2017 Challenges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scattering. Here, Nguyen et al. develop a quantitative phase method that uses low-coherence interferometry for label-free 3D imaging in scattering ...
Tan H. Nguyen +4 more
doaj +4 more sources
Label-Free Single Cell Viability Assay Using Laser Interference Microscopy [PDF]
Biology, 2021 Laser interference microscopy (LIM) is a promising label-free method for single-cell research applicable to cell viability assessment in the studies of mammalian cells.
Yulia Beloglazova +6 more
doaj +3 more sources
Single-shot isotropic differential interference contrast microscopy
Nature Communications, 2023 Differential interference contrast (DIC) microscopy allows high-contrast, low-phototoxicity, and label-free imaging of transparent biological objects, and has been applied in the field of cellular morphology, cell segmentation, particle tracking, optical
Xinwei Wang +18 more
doaj +3 more sources
Epi-illumination gradient light interference microscopy for imaging opaque structures [PDF]
Nature Communications, 2019 Quantitative phase imaging techniques have been limited by multiple scattering of light or its use in transmission mode. Here, the authors show a gradient light interference microscopy method in a reflection geometry which allows for label-free phase ...
Mikhail E. Kandel +10 more
doaj +3 more sources
Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy [PDF]
Nature Communications, 2018 3D super-resolution imaging of dynamic processes in live cells is still challenging, especially in a large field of view. Here the authors combine SIM with multi-angle evanescent light illumination and achieve improved lateral and axial resolution, with ...
Youhua Chen +14 more
doaj +3 more sources
An acquisition and analysis pipeline for scanning angle interference microscopy [PDF]
Nature Methods, 2016 To the Editor We describe open-source software and hardware tools for calibrating, acquiring, and analyzing images for scanning angle interference microscopy (SAIM) analysis.
Catherine B Carbone +2 more
exaly +3 more sources
Label-free quantitative evaluation of breast tissue using Spatial Light Interference Microscopy (SLIM) [PDF]
Scientific Reports, 2018 Breast cancer is the most common type of cancer among women worldwide. The standard histopathology of breast tissue, the primary means of disease diagnosis, involves manual microscopic examination of stained tissue by a pathologist.
Hassaan Majeed +4 more
doaj +3 more sources
Spatial light interference microscopy: principle and applications to biomedicine. [PDF]
Adv Opt Photonics, 2021 In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination.
Chen X, Kandel ME, Popescu G.
europepmc +2 more sources