Results 61 to 70 of about 95,522 (262)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Analysis of the Represented Interior Space: Hermeneutic Study of the Peres Maldonado Ex-voto

open access: yesInteriority
The paper demonstrates the application of Henri Lefebvre's postulates in the hermeneutical study of the Peres Maldonado Ex-voto, created in Aguascalientes in 1777.
Blanca Ruiz Esparza Díaz de León   +1 more
doaj   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

open access: yesFEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph   +7 more
wiley   +1 more source

Strong Hydro‐Related Localized Long‐Period Crustal Deformation Observed in the Plate Boundary Observatory Borehole Strainmeters

open access: yesGeophysical Research Letters, 2018
Strong localized hydro‐related long‐period strain variations, with amplitude up to about 2,000 ns (nanostrain) (1–2 orders of magnitude larger than the tidal deformation), are observed in some Plate Boundary Observatory borehole strainmeters in Parkfield
Zhou Lu, Lianxing Wen
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Public Space as Interior and Interior as Public Space [PDF]

open access: yesCivil Engineering Research Journal, 2021
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

Analysis of the regenerative design of Ru porcelain in hotels

open access: yesAl-Qadisiyah Journal for Engineering Science
Ru porcelain is part of China’s traditional culture, and in recent years, the use of Ru porcelain as a design element of hotel space has gradually increased. The primary purpose of this study is to explore the integration of Ru porcelain and contemporary
Cui   +3 more
doaj   +1 more source

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