Results 91 to 100 of about 20,074,763 (285)

Molecular markers, analysis and the population genetics of parasites [PDF]

, 2002
In this study different molecular techniques are contrasted (RAPD's, allozyme, sequencing mtDNA, sequencing ribosomal spacers) and appropriate analytical methods (allelic and infinite-sites approaches; inbreeding and coalescent models) used for ...
Constantine, Clare Colleen
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Phylogenetics of Cucumis (Cucurbitaceae) [PDF]

, 2007
Background: Melon, Cucumis melo, and cucumber, C. sativus, are among the most widely cultivated crops worldwide. Cucumis, as traditionally conceived, is geographically centered in Africa, with C. sativus and C.
Kocyan, Alexander, Schaefer, Hanno, Renner, Susanne S.   +2 more
core   +1 more source

Enhanced DNA barcode datasets for Aristida grasses: new whole genome skimming data for the protected species Aristida triseta Keng integrated with public sequencesScience Data BankNCBI BiosampleGenBankINSDC repositoriesINSDC repositories

Data in Brief
Aristida triseta Keng is a Class II national protected wild plant in China, possessing significant ecological and economic importance. In this study, the complete chloroplast genome of A. triseta was sequenced using the DNBSEQ-T7 platform.
Xiaoman Wang, Kai Mu, Chao Xu, Zhengge Zhu, Xueying Yang, Jin Zhang   +5 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Genetic Relationships of Mulberry (Morus l.) Using Internal Transcribed Spacer (ITS) Markers

, 2021
Mulberry, belonging to the family Moraceae, and genus Morus. Thirty-nine accessions, which belong to 9 species of Morus and 2 species as outgroup (Ficus adhatodifolia, Broussonetia papyrifera) were examined using ITS markers in this study. Genetic variation and phylogenetic relationships among 19 samples from West Sumatra were assessed using the ...
Pratiwi, Indah Rahayu, Syamsuardi, Nurainas   +2 more
openaire   +2 more sources

Identification of Common Edible and Medicinal Mushrooms by DNA Barcoding

World Journal of Traditional Chinese Medicine, 2016
Objective: To identify common edible and medicinal mushrooms using the internal transcribed spacer (ITS) region.
Yan-Yan Su, Huan Tang, Xi-Wen Li, Wei Sun, Zeng-Hua Chen, Li Xiang   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Lactarius deliciosus isolate UEZB1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence [PDF]

, 2012
LOCUS JQ066791 439 bp DNA linear PLN 20-MAR-2012 DEFINITION Lactarius deliciosus isolate UEZB1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed ...
Martins, Maria Rosário, Louro, Rogério, Ganhão, Elsa, Ragonezi, Carla, Klimaszewska, Kristina, Zavattieri, Amely, Santos-Silva, Celeste, Caldeira, Ana Teresa   +7 more
core  

Phân lập giống nấm mối Termitomyces clypeatus

Tạp chí Khoa học Đại học Cần Thơ, 2016
Mẫu nấm mối dùng để phân lập được thu hái ở xã An Linh huyện phú Giáo và xã Định Hiệp, huyện Dầu Tiếng, tỉnh Bình Dương. Phương pháp nuôi cấy mô quả thể nấm đã được sử dụng để tạo nguồn giống thuần khiết trong ống nghiệm. Quá trình phân lập nấm mối được
Nguyễn Thị Ngọc Nhi, Trần Nhân Dũng   +1 more
doaj   +1 more source