Results 121 to 130 of about 20,074,763 (285)

Mycena luxaustralis , a new bioluminescent species in section Sacchariferae from southern Chile

Plant and Fungal Systematics
Bioluminescent fungi emit light through chemical luminescence of fungal tissues. More than 100 species of bioluminescent fungi have been described in five distinct groups of Agaricales : Omphalotus , Armillaria , the Lucentipes lineage within ...
Pablo Sandoval-Leiva, Adriana Calle, Nicolas Niveiro, Benjamin Lemmond, Matthew E. Smith   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Maximum likelihood tree of Oedogonium internal transcribed spacer (ITS) sequence data.

, 2014
Maximum likelihood tree of Oedogonium internal transcribed spacer (ITS) sequence data (scale at bottom). Numbers near each node refer to bootstrap support values, nodes with
Stephen Skinner (531970), Nicholas A. Paul (165653), Rocky de Nys (165661), Rebecca J. Lawton (414594)   +3 more
core   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Comparison of PCR-RFLP pattern with sequencing analysis of the ITS region of Hyrcanain\'s Tilia

تاکسونومی و بیوسیستماتیک, 2014
Sequencing of ITS region is one of the candidate markers as a DNA barcoding in Plants. Since sequencing technique is expensive and time consuming, we used PCR-RFLP technique and compared the result of these methods (PCR-RFLP and sequencing) to evaluate ...
Hamed Yousefzadeh, Hamid Bina, Abasalt Hosseinzadeh Colagar   +2 more
doaj  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Comparison of commonly used software pipelines for analyzing fungal metabarcoding data

BMC Genomics
Background Metabarcoding targeting the internal transcribed spacer (ITS) region is commonly used to characterize fungal communities of various environments. Given their size and complexity, raw ITS sequences are necessarily processed and quality-filtered
Theresa Rzehak, Nadine Praeg, Giulio Galla, Julia Seeber, Heidi Christine Hauffe, Paul Illmer   +5 more
doaj   +1 more source

Cotton leaf blight disease caused by Alternaria alternata in Sudan

Journal of Plant Protection Research, 2019
Genetically modified Bt cotton (Gossypium hirsutum) leaves with typical symptoms of Alternaria early blight disease resembling that of tomato and potato were observed in the main cotton growing schemes in Sudan.
Omyma Elmahi Mohamed, Mayada Mamoun Beshir, Nafisa Elmahi Ahmed   +2 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source