Results 41 to 50 of about 40,065 (255)

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA

AMB Express, 2017
The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ...
María Teresa Alvarez-Zúñiga, Alejandro Santiago-Hernández, Johan Rodríguez-Mendoza, Jorge E. Campos, Patricia Pavón-Orozco, Sergio Trejo-Estrada, María Eugenia Hidalgo-Lara   +6 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Characterizing Crustose Lichen Communities—DNA Metabarcoding Reveals More than Meets the Eye

Diversity, 2022
Biodiversity inventories are important for informing land management strategies, conservation efforts, and for biomonitoring studies. For many organismal groups, including lichens, comprehensive, accurate inventories are challenging due to the necessity ...
Jacob R. Henrie, Brenden M. Thomson, Andrew August Yungfleisch, Michael Kerr, Steven D. Leavitt   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Phylogeny Study of Genus Pelvetia in Korea by Internal Transcribed Spacer Sequence (ITS) [PDF]

Journal of Life Science, 2009
The brown algae, or phaeophytes, are a large group of multicellular algae, including many notable types of seaweed. We analysed intra- and interspecific phylogenic studies within the genus Pelvetia in Korea and compared them with results of both same and different species in GenBank. The sequences for P.
openaire   +1 more source

ITSxpress version 2: software to rapidly trim internal transcribed spacer sequences with quality scores for amplicon sequencing

Microbiology Spectrum
The nuclear ribosomal RNA (rRNA) internal transcribed spacer (ITS) regions are commonly used to identify fungi and other eukaryotic taxa in amplicon sequencing.
Sveinn V. Einarsson, Adam R. Rivers
doaj   +1 more source

Development of a real-time LAMP assay for monofloral honey authentication using rape honey

CyTA - Journal of Food, 2020
The objective of this study was to establish a real-time LAMP assay for authentication of rape (Brassica napus) honey to protect consumers from commercial honey adulteration.
Yongzhen Wang, Meng Zhang, Deguo Wang, Yongqing Zhang, Xuexue Jiao, Yanhong Liu   +5 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source