Results 101 to 110 of about 1,572 (185)

Design and optimisation of rapid real‐time PCR assays for the detection of key Culicoides species

open access: yesMedical and Veterinary Entomology, EarlyView.
Species‐specific real‐time PCR assays were designed and optimised using primer/probe sets targeting C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi, C. pulicaris, C. punctatus and C. impunctatus. Primer specificity and amplification efficiency were validated through in silico specificity analysis and real‐time PCR amplification profiling.
Elsie Isiye   +10 more
wiley   +1 more source

Eco‐evolutionary context modifies a destructive plant invader's response to climate

open access: yesNew Phytologist, EarlyView.
Abiotic environment–fitness relationships can be shaped by evolutionary, ecological, and eco‐evolutionary contexts. Summary Understanding the relationship between climate and fitness will be important when predicting how plant populations respond to climate change. We conducted a replicated common garden experiment (4 sites × 2 yr) with 96 genotypes (n 
Megan L. Vahsen   +29 more
wiley   +1 more source

Cell‐Specific Expression and Cellular Compartmental Regulation in Camptothecin Biosynthesis

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Plant secondary metabolites such as monoterpenoid indole alkaloids (MIAs) show tightly regulated biosynthesis and accumulation in specific organelles of distinct cell types. However, the cell‐specific expression patterns of anticancer MIA camptothecin biosynthetic genes in Ophiorrhiza pumila and the regulatory mechanisms of camptothecin ...
Xiaolong Hao   +12 more
wiley   +1 more source

High‐Contiguity Haplotype‐Resolved Genome Assembly of the Hexaploid Actinidia valvata Rootstock Sheds Light on Waterlogging Resistance Gene

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Polyploidisation, creating redundant or diverged copies of the genome, is a major driving force in plant evolution, diversification and environmental adaptation, including for the kiwifruit genus (Actinidia Lindl.). We present a high‐contiguity, haplotype‐resolved genome assembly of the hexaploid Actinidia valvata rootstock cultivar ...
Miaomiao Lin   +11 more
wiley   +1 more source

Directed Evolution of Plant‐Associated Bacteria Enhances Plant Holobiont Stress Tolerance

open access: yesPlant, Cell &Environment, EarlyView.
ABSTRACT Plant stress responses are shaped by both the plant genome and its associated microbial communities, which together form the plant holobiont. Given the rapid adaptive potential of plant‐associated microbes, we hypothesised that directed evolution under selective pressure can accelerate the development of holobiont‐level stress tolerance ...
Mohammadhossein Ravanbakhsh, Wu Xiong
wiley   +1 more source

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