Design and optimisation of rapid real‐time PCR assays for the detection of key Culicoides species
Species‐specific real‐time PCR assays were designed and optimised using primer/probe sets targeting C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi, C. pulicaris, C. punctatus and C. impunctatus. Primer specificity and amplification efficiency were validated through in silico specificity analysis and real‐time PCR amplification profiling.
Elsie Isiye +10 more
wiley +1 more source
Neural and behavioral responses to reproductive signals in male chorus frogs. [PDF]
Ochoa CB +3 more
europepmc +1 more source
Eco‐evolutionary context modifies a destructive plant invader's response to climate
Abiotic environment–fitness relationships can be shaped by evolutionary, ecological, and eco‐evolutionary contexts. Summary Understanding the relationship between climate and fitness will be important when predicting how plant populations respond to climate change. We conducted a replicated common garden experiment (4 sites × 2 yr) with 96 genotypes (n
Megan L. Vahsen +29 more
wiley +1 more source
Root morphogenetic responses to waterlogging stress in plants: from structural reconfiguration to molecular regulatory mechanisms. [PDF]
Sha S +5 more
europepmc +1 more source
Cell‐Specific Expression and Cellular Compartmental Regulation in Camptothecin Biosynthesis
ABSTRACT Plant secondary metabolites such as monoterpenoid indole alkaloids (MIAs) show tightly regulated biosynthesis and accumulation in specific organelles of distinct cell types. However, the cell‐specific expression patterns of anticancer MIA camptothecin biosynthetic genes in Ophiorrhiza pumila and the regulatory mechanisms of camptothecin ...
Xiaolong Hao +12 more
wiley +1 more source
The Influence of Habitat Heterogeneity and Human Disturbance on Trophic Interactions of <i>Sebastiscus marmoratus</i> and <i>Scorpaenopsis cirrosa</i> in Wanshan Archipelago. [PDF]
Xie H +11 more
europepmc +1 more source
ABSTRACT Polyploidisation, creating redundant or diverged copies of the genome, is a major driving force in plant evolution, diversification and environmental adaptation, including for the kiwifruit genus (Actinidia Lindl.). We present a high‐contiguity, haplotype‐resolved genome assembly of the hexaploid Actinidia valvata rootstock cultivar ...
Miaomiao Lin +11 more
wiley +1 more source
How <i>Drosophila suzukii</i> Acquires and Interacts with Its Microbiome Across Ecological Contexts. [PDF]
Ernstberger H, Palmieri G, Sun JS.
europepmc +1 more source
Directed Evolution of Plant‐Associated Bacteria Enhances Plant Holobiont Stress Tolerance
ABSTRACT Plant stress responses are shaped by both the plant genome and its associated microbial communities, which together form the plant holobiont. Given the rapid adaptive potential of plant‐associated microbes, we hypothesised that directed evolution under selective pressure can accelerate the development of holobiont‐level stress tolerance ...
Mohammadhossein Ravanbakhsh, Wu Xiong
wiley +1 more source
Investigating interspecific mating in the thelytokous predatory mite Amblyseius herbicolus (Chant) (Acari: Phytoseiidae), with comparative observations from three sexually reproducing phytoseiid species. [PDF]
Zhang K, Cao J, Li X, Zhang ZQ.
europepmc +1 more source

