Human Proteoglycan Linkage Region Glycosyltransferases are Dimeric and Show Unexpected Specificities. [PDF]
Angew Chem Int Ed EnglNon‐canonical glycopeptides of the proteoglycan linkage region are accessible by the enzymes B3GalT6 and GlcAT‐1 confirming a recently discovered rescue mode in glycosaminoglycan (GAG) biosynthesis. The crystal structure of B3GalT6 revealed a covalent dimer linked by a disulfide. Abstract We selected the N,O‐glycosylated proteoglycan bikunin as a model
Weidler S +16 more
europepmc +3 more sources
Amide and Thioester Synthesis Via Oxidative Coupling of Alcohols with Amines or Thiols Using Alcohol Dehydrogenases. [PDF]
Angew Chem Int Ed EnglAlcohol dehydrogenases (ADHs) are best known for reducing ketones to chiral alcohols, but their oxidative potential is rarely exploited. Here, we show that selected ADHs catalyze newly discovered promiscuous transformations: the oxidative coupling of primary alcohols with amines or thiols, enabling the direct and efficient synthesis of a broad range of
Damian M +4 more
europepmc +3 more sources
The mucin-degradation strategy of Ruminococcus gnavus:The importance of intramolecular trans-sialidases [PDF]
, 2016 We previously identified and characterized an intramolecular trans-sialidase (IT-sialidase) in the gut symbiont Ruminococcus gnavus ATCC 29149, which is associated to the ability of the strain to grow on mucins. In this work we have obtained and analyzed
Crossman, Lisa C. +6 more
core +2 more sources
GENETICS AND BIOCHEMISTRY OF DEHALOGENATING ENZYMES [PDF]
, 1994 Microorganisms that can utilize halogenated compounds as a growth substrate generally produce enzymes whose function is carbon-halogen bond cleavage. Based on substrate range, reaction type and gene sequences, the dehalogenating enzymes can be classified
Janssen, Dick B +2 more
core +5 more sources
Characterization of antioxidant olive oil biophenols by spectroscopic methods [PDF]
, 2010 Olive oil contains numerous phenolic components with well-recognized health-beneficial activity. The major phenolic compounds present in olives and virgin olive oil-hydroxytyrosol, oleuropein and the oleuropein aglycones 3,4-DHPEA-EA and 3,4-DHPEA-EDA-as
Calheiros, Rita +3 more
core +1 more source
Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy [PDF]
, 2011 Increasing insights into the involvement of endogenous lectins in disease processes fuel the interest to develop potent inhibitors. As a consequence, robust assay procedures are required. Due to their activity as adhesion/growth-regulatory effectors this
André, Sabine +5 more
core +1 more source
Adaptive HIV-1 evolutionary trajectories are constrained by protein stability [PDF]
, 2017 Despite the use of combination antiretroviral drugs for the treatment of HIV-1 infection, the emergence of drug resistance remains a problem. Resistance may be conferred either by a single mutation or a concerted set of mutations.
Kandathil, Shaun M. +3 more
core +2 more sources
Members of the chloride intracellular ion channel protein family demonstrate glutaredoxin-like enzymatic activity [PDF]
, 2015 © 2015 Al Khamici et al. The Chloride Intracellular Ion Channel (CLIC) family consists of six evolutionarily conserved proteins in humans. Members of this family are unusual, existing as both monomeric soluble proteins and as integral membrane proteins ...
Brown, LJ +11 more
core +1 more source
The immune‐related, TGA1 redox‐switch: to be or not to be?
, 2020 New Phytologist, EarlyView.
Yuan Li, Gary J. Loake
wiley +1 more source
Repetitive and Non-Repetitive DNA Sequences and a Speculation on the Origins of Evolutionary Novelty [PDF]
, 1971 Recent experimental information on DNA sequence repetition is reviewed, and the significance of both repetitive and non-repetitive sequence considered.
Britten, Roy J., Davidson, Eric H.
core +1 more source