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Ion-Exchange Chromatography

1979
Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Ion-Exchange Chromatography of Proteins

2003
Ion-exchange chromatography is the most widely used technique in protein chromatography (1). This is because it is nearly always possible to develop successful ionexchange separations for proteins, and the materials required are relatively inexpensive.
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Analytical ion exchange chromatography of proteins

Analytical Biochemistry, 1982
A low-pressure system for analytical ion exchange chromatography of proteins is described. Elution of proteins is performed by salt gradient and protein concentration is monitored at 206 nm. Protein mixtures consisting of microgram amounts of each component can be quantitatively resolved in 40 min.
Michael Laskowski, Wojciech Ardelt
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Chromatography of xanthines on ion-exchange resins

Journal of Chromatography A, 1979
Short columns of a 4% crosslinked cation-exchange resin gave good chromatography of xanthines, including caffeine, theophylline and hypoxanthine, and related polar aromatic compounds. Elution volumes and sequences can be modified by changing pH, solvent composition and resin counter-ion.
G.Alan Eiceman   +2 more
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Ion exchange chromatography of antibody fragments

Biotechnology and Bioengineering, 2006
AbstractEffects of pH and conductivity on the ion exchange chromatographic purification of an antigen‐binding antibody fragment (Fab) of pI 8.0 were investigated. Normal sulfopropyl (SP) group modified agarose particles (SP Sepharose™ Fast Flow) and dextran modified particles (SP Sepharose XL) were studied.
James M. Van Alstine   +6 more
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Adsorption, Chromatography, Ion Exchange

2011
Adsorption is the loading of solid surfaces with substances present in a surrounding fluid phase or, in other words, it is a surface effect between a solid and a fluid phase. Sometimes molecules of the fluid phase are not only fixed on the surface but can additionally enter the bulk of the nonporous solid phase according to a volume effect.
Alfons Mersmann   +2 more
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Chromium speciation at trace level in potable water using hyphenated ion exchange chromatography and inductively coupled plasma mass spectrometry with collision/reaction interface

, 2010
A simple method is described for chromium speciation at sub-µg L−1 level in potable water samples using hyphenated ion exchange chromatography (IEC) and inductively coupled plasma mass spectrometry (ICP-MS) with collision/reaction interface (CRI).
Liyan Xing, D. Beauchemin
semanticscholar   +1 more source

Analysis by Chromatography and Ion Exchange

Nature, 1954
Inorganic Chromatography By Orsino C. Smith. Pp. x + 134 + 5 plates. (New York: D. Van Nostrand Company, Inc.; London: Macmillan and Co., Ltd., 1953.) 37s. 6d. net. Chromatographic Methods of Inorganic Analysis With Special Reference to Paper Chromatography. By Dr. F. H. Pollard and Dr. J. F. W. McOmie. Pp. viii + 192 + 4 plates.
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Ion-exchange chromatography of tissue nucleotides

Journal of Chromatography A, 1969
Abstract A procedure has been devised for the isolation of soluble tissue nucleotides by ion-exchange chromatography on 1.0 × 90 cm columns of DEAE-Sephadex A-25, acetate form, using consecutive concave concentration gradients of the volatile salt triethylammonium acetate at pH 4.7.
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Superficial Ion-exchange Chromatography

Nature, 1965
RECENTLY the techniques of gas chromatography have been applied to chromatography in the liquid phase to separate small quantities of mixtures very rapidly. Techniques have been described for adsorption chromatography1, partition chromatography2, and ion-exchange chromatography3 where the inner surface of a capillary tube was coated with a liquid ion ...
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