Results 71 to 80 of about 99 (97)

Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this
Juan J. Romero, Eleanor W. Jenkins, Jacob I. Monroe, S. Ranil Wickramasinghe, Xianghong Qian, Dibakar Bhattacharyya, Scott M. Husson   +6 more
wiley   +1 more source

In situ biomimetic materials for dentin repair

BMEMat, EarlyView.
This review primarily discusses the role and clinical application of key biomimetic materials for in situ dentin repair, providing a feasible foundation for future material and performance optimization in situ dentin regeneration. Abstract Dental defects, ranking among the most prevalent diseases globally, pose a serious threat to human health, with ...
Jingjing Deng, Siyi Ren, Xuliang Deng, Yan Wei   +3 more
wiley   +1 more source

Optimizing biophysical properties of cellular niches to enhance stem cell‐derived extracellular vesicle function in musculoskeletal regeneration

BMEMat, EarlyView.
Extracellular vesicles (EVs) derived from stem cells show promise for applications in regenerative medicine, but their scalability and yield remain challenges. This review explores the approaches for biophysical modulations within cell niches on EV properties, discusses the current clinical application of EVs, and provides an outlook on the existing ...
Yang Xu, Chung Yin Matthew Cheung, Liling Liu, Hoi Pan Harry Cheung, Kam Yu Tam, Dai Fei Elmer Ker, Sarah H. Cartmell, Chuanbin Mao, Zhiyong Zhang, Dan Michelle Wang   +9 more
wiley   +1 more source

Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes

Biotechnology Progress, Volume 41, Issue 2, March/April 2025.
Abstract The challenging task of designing biopharmaceutical downstream processes is initially to select the type of unit operations, followed by optimizing their operating conditions. For complex flowsheet optimizations, the strategy becomes crucial in terms of duration and outcome.
Daphne Keulen, Myrto Apostolidi, Geoffroy Geldhof, Olivier Le Bussy, Martin Pabst, Marcel Ottens   +5 more
wiley   +1 more source

pH and conductivity transients during elution of IgG from protein A columns

Biotechnology Progress, EarlyView.
Abstract The evolution of pH and conductivity waves during elution of IgG from protein A columns is studied for the resin MabSelect PrismA as well as other commercial resins using glycine and acetate buffers as eluents. The effects of buffer composition, IgG load, and residence time are explored.
Rainer Hahn, Lukas Berger, Jürgen Beck, Giorgio Carta   +3 more
wiley   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

Biotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi, Mary Otto, Corey Kretzmer, Amber Petersen, Channing McLaurin, Jana Mahadevan, Jason Gustin, Trissa Borgschulte, David Razafsky   +8 more
wiley   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

Biotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold, Sheridan Waldack, Grace James, Trisha Colling, Lynn Manchester, Taravat Sarvari, Amanda Bekkala, Seth A. Kriz, Madison Baldwin, Emily Agustin‐Mazariegos, Michael J. Betenbaugh, Caryn L. Heldt   +11 more
wiley   +1 more source

Mining Thermophile Genomes for New PETases with Exceptional Thermostabilities Using Sequence Similarity Networks

ChemBioChem, EarlyView.
Targeted genome mining identified thermostable enzymes (PETases) that hydrolyze the commonly used plastic polymer polyethylene terephthalate (PET). Thermostable enzymes are identified from thermophilic microbial sources. Experimental results reveal enzymatic activity on authentic PET films, highlighting potential for industrial application.
Zhenyu Hu, Kody Klupt, David L. Zechel, Zongchao Jia, Graeme Howe   +4 more
wiley   +1 more source

Covalent Peptide‐Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects

ChemBioChem, EarlyView.
The three peptides RGD, DGEA, and KKGHK are covalently attached to the 2D nanomaterial graphene oxide (GO) to create new biomaterials for the treatment of severe bone injuries. These GO modifications improve cell spreading, stem cell differentiation into bone cell types, and formation of blood vessel‐like structures.
Michelle E. Wolf, Yaxuan Liu, Jason D. Orlando, Jingzhi Zhou, Stefanie A. Sydlik   +4 more
wiley   +1 more source

A de novo designed metalloprotein displays variable thermal stability and binding stoichiometry with transition metal ions

ChemBioChem, Accepted Article.
Metal‐binding selectivity in natural proteins is determined by multiple factors such as the protein’s structure, metal concentration within cellular compartments and the presence of metallochaperones. The in vitro selectivity of proteins for transition metal ions is largely governed by the Irving‐Williams series, that states protein‐metal complex ...
Britt Rooijakkers, Gaya Verhagen, Anneloes Cramer-Blok, Ed Zuidinga, Aimee Lynn Boyle   +4 more
wiley   +1 more source