Results 41 to 50 of about 9,827,022 (250)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

ZnO Nanowire Networks as Photoanode Model Systems for Photoelectrochemical Applications

Nanomaterials, 2018
In this work, the fabrication of zinc oxide (ZnO) nanowire networks is presented. By combining ion-track technology, electrochemical deposition, and atomic layer deposition, hierarchical and self-supporting three-dimensional (3D) networks of pure ZnO ...
Liana Movsesyan, Albert Wouter Maijenburg, Noel Goethals, Wilfried Sigle, Anne Spende, Florent Yang, Bernhard Kaiser, Wolfram Jaegermann, Sun-Young Park, Guido Mul, Christina Trautmann, Maria Eugenia Toimil-Molares   +11 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

FEATURES OF FORMING OF COPPER DEPOSIT IN PORES OF SILICON OXIDE

Весці Нацыянальнай акадэміі навук Беларусі: Серыя фізіка-тэхнічных навук, 2016
Using the SiO2 porous templates on silicon substrate, Si/SiO2(Cu) nanostructures have been synthesized by electrochemical method. A comprehensive study by means of scanning electron, transmission electron, atomic force microscopy and diffraction analysis
E. Yu. KANIUKOV, E. K. BELONOGOV, D. V. YAKIMCHUK, A. L. KOZLOVSKIY, K. K. KADYRZHANOV, G. M. ARZUMANYAN, S. E. DEMYANOV   +6 more
doaj  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Development and beam test of a continuous wave radio frequency quadrupole accelerator

Physical Review Special Topics. Accelerators and Beams, 2012
The front end of any modern ion accelerator includes a radio frequency quadrupole (RFQ). While many pulsed ion linacs successfully operate RFQs, several ion accelerators worldwide have significant difficulties operating continuous wave (CW) RFQs to ...
P. N. Ostroumov, B. Mustapha, A. Barcikowski, C. Dickerson, A. A. Kolomiets, S. A. Kondrashev, Y. Luo, D. Paskvan, A. Perry, D. Schrage, S. I. Sharamentov, R. Sommer, W. Toter, G. Zinkann   +13 more
doaj   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Alternative genome sequencing approaches of SARS-CoV-2 using Ion AmpliSeq Technology

MethodsX
A thorough understanding of SARS-CoV-2 genetic features is compulsory to track the ongoing pandemic across multiple geographical locations of the world.
Md. Shazid Hasan, M. Shaminur Rahman, Prosanto Kumar Das, A.S.M. Rubayet Ul Alam, Ovinu Kibria Islam, Hassan M. Al-Emran, M. Anwar Hossain, Iqbal Kabir Jahid   +7 more
doaj   +1 more source

Field Report from Collaborative Research Center 1625: Heterogeneous Research Data Management Using Ontology Representations

Advanced Engineering Materials, EarlyView.
A unified research data management framework for heterogeneous materials data is presented. The system integrates multimodal datasets using ontologies and knowledge graphs, enabling interoperability and FAIR (findable, accessible, interoperable, reusable) data principles. By linking data across scales and workflows, it supports reproducible, Artifitial
Doaa Mohamed, Samuel García Vázquez, Behnam Mardani, Victor Dudarev, Alfred Ludwig, Maribel Acosta, Markus Stricker   +6 more
wiley   +1 more source