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1979
Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Antibody Purification: Ion-Exchange Chromatography
2009Ion exchange chromatography techniques are the focus of this chapter and they showcase the power of this method for the purification of proteins and monoclonal antibodies. The technique is powerful and can separate biomolecules that have minor differences in their net charge, e.g., two protein molecules differing by a single charged amino acid.
Ana Cristina, Grodzki, Elsa, Berenstein
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Ion Exchange in Paper Chromatography
Nature, 1953IT has been shown by Schute that several mixtures of alkaloids can be separated by chromatography on paper using aqueous ammonia1. Because the chromatograms have the characteristics of a partition chromatogram, it is probable that the above separation is due to partition between an aqueous imbibition phase and an aqueous mobile phase.
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Ion-Exchange Chromatography in Alcohol
Nature, 1955IN the technique of ion-exchange chromatography, the order in which compounds are eluted from a column depends basically upon differences in their ionization constants; but other factors modify and in many instances reverse the expected order1. This is illustrated in Fig.
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Ion-Exchange Chromatography of Proteins
2003Ion-exchange chromatography is the most widely used technique in protein chromatography (1). This is because it is nearly always possible to develop successful ionexchange separations for proteins, and the materials required are relatively inexpensive.
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ION EXCHANGE | Ion Chromatography Instrumentation
2005Ya.I. Yashin, A. Ya Yashin, H.F. Walton
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ION EXCHANGE | Chelation Ion Chromatography
2005Pavel N. Nesterenko, Phil Jones
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