Results 91 to 100 of about 456,934 (299)
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
International Journal of Electrochemical Science, 2012 The generation and long-term stability of superoxide ion (O2•-) in two trifluoromethanesulfonate [TfO]-anion-based ionic liquids (ILs), with cations of 1-butyl-1-methylpyrrolidinium [BMPyrr]+ and 1-butyl-2,3-dimethylimidazolium [BDMIm]+ have been ...
Maan Hayyan +3 more
doaj +1 more source
ESTERIFICATION IN IONIC LIQUIDS
Chemical Engineering Transactions, 2009 Not available.
Deyan Naydenov, Hans-Joerg Bart
openaire +2 more sources
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Ionic Liquids as high energy propellants
Ionic liquids are receiving an ever-increasing interest due to their unique properties and are also under investigation in the field of energetic materials. Potential applications are gun and liquid rocket propellants.
Weiser, Volker +3 more
core +1 more source
Application of Ionic Liquids for Chemical Demulsification: A Review
, 2020 In recent years, ionic liquids have received increasing interests as an effective demulsifier due to their characteristics of non-flammability, thermal stability, recyclability, and low vapor pressure.
Nahid Hassanshahi +5 more
core +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source