Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples. [PDF]
A triplex real-time (TRT-PCR) assay was developed to ensure a rapid and reliable detection of Mycobacterium avium subsp. paratuberculosis (Map) in faecal samples and to allow routine detection of Map in farmed livestock and wildlife species.
Walravens, Karl +7 more
core +1 more source
Little data concerning the distribution of Mycobacterium avium ssp. paratuberculosis (MAP) in zoological gardens is available. The presence of MAP in captured wildlife might provide further information on non-ruminant hosts and expand the list of animals susceptible to MAP being potential sources of MAP transmission.
Muenster, Pia +4 more
openaire +3 more sources
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp.
Sidi-Boumedine, Karim +8 more
core +4 more sources
Detection of Mycobacterium avium subspecies paratuberculosis in dairy cattle from Shahrekord, Iran
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of an incurable intestinal disease in ruminants commonly known as Johne’s disease (JD).
M. Rahmani Dehkordi +4 more
doaj +1 more source
The aim of this study was to investigate the occurrence of subclinical Mycobacterium avium spp. paratuberculosis (MAP) infections at slaughter by testing ileocaecal lymph nodes with a semi-nested IS900 PCR.
Pia Münster +19 more
core +1 more source
MAP morphotypes survive 70°C and are positive for MAP 16SrRNA and IS900.
(A) MAP K-10 log phase and spores, B. subtilis and C. perfringens were heat treated at 70°C for 30 min and subsequently treated with 2% lysozyme, PK, or kanamycin.
Don Sanjiv Ariyakumar (334290) +4 more
core +1 more source
Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds. [PDF]
Beinhauerova M, Slana I.
europepmc +1 more source
IS1626, a new IS900-related Mycobacterium avium insertion sequence
An insertion sequence designated IS1626 was isolated and characterized from a Mycobacterium avium clinical strain. IS1626 was detected by high-stringency hybridization with the pMB22/S12 probe from IS900 of Mycobacterium paratuberculosis.
Corey Pawlichuk +4 more
core
Improved DNA Amplification of the Hallmark IS900 Element in Mycobacterium avium subsp. paratuberculosis: a Reexamination Based on Whole-Genome Sequence Analysis. [PDF]
Bannantine JP +3 more
europepmc +1 more source
Mycobacterial Interspersed Repeat Unit-Variable Number Tandem Repeat Typing of Mycobacterium avium Strains Isolated from the Lymph Nodes of Free-Living Carnivorous Animals in Poland. [PDF]
Orłowska B +11 more
europepmc +1 more source

