A prospective microbiological surveillance in a teaching Italian Hospital; microbial isolations and in vitro antimicrobial susceptibility levels, and their modifications over time [PDF]
Roberto Manfredi
openalex +1 more source
Abstract We present the first use of a bioengineered mammalian transposase system derived from Myotis lucifugus (bMLT) for integration of expression vectors into the CHO genome, focusing on GFP and trastuzumab production. Initially, CHO‐K1 cells are transfected with a GFP reporter and varying amounts of bMLT DNA or mRNA.
Nicolas Marx+10 more
wiley +1 more source
The Story behind the Science: On the discovery of respiratory syncytial virus. [PDF]
Casadevall A+3 more
europepmc +1 more source
A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coli
Abstract Variable lymphocyte receptors (VLRs) are the antigen receptors of jawless vertebrates such as lamprey. VLRs are of growing biotechnological interest for their ability to bind certain antigenic targets with higher affinity than traditional immunoglobulins.
Elizabeth A. Appelt+9 more
wiley +1 more source
Infection model of THP-1 cells, growth dynamics, and antimicrobial susceptibility of clinical Mycobacterium abscessus isolates from cystic fibrosis patients: Results from a multicentre study. [PDF]
Ruedas-López A+8 more
europepmc +1 more source
Abstract The production of disulfide‐containing recombinant proteins often requires refolding of inclusion bodies before purification. A pre‐refolding purification step is crucial for effective refolding because impurities in the inclusion bodies interfere with refolding and subsequent purification.
Shuichiro Kimura+4 more
wiley +1 more source
Reducing blood culture contamination: an environmental imperative. [PDF]
Gregg S, Purcell N, Doyle M, Chan G.
europepmc +1 more source
Improving outcomes in intensified processing via optimization of the cell line development workflow
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi+8 more
wiley +1 more source
Analysis of Drug-Resistant Bacteria Seasonality in Japan Using Financial Time Series Analysis Method: A Nationwide Longitudinal Study. [PDF]
Ito H, Oshida J, Fujita M, Kobayashi D.
europepmc +1 more source