Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Draft genome of <i>Rhodococcus</i> sp. no. 34, a soil isolate capable of curcumin degradation. [PDF]
Hashimoto Y +3 more
europepmc +1 more source
Dynamics of natural killer cell function upon recurrent stimulation
Weekly stimulation of peripheral blood‐derived NK cells with engineered K562 feeder cells resulted in two distinct growth phases, shifting around Day 21–26. Cells in growth phase 2 exhibited slower growth, reduced metabolic activity, and lower cytotoxicity than cells from growth phase 1, which has implications for NK cell biomanufacturing.
Jennifer One +4 more
wiley +1 more source
Draft genome sequence of endometrial <i>Alloscardovia omnicolens</i> strain Y28 identified in endometrial cancer. [PDF]
Anwyl GA +3 more
europepmc +1 more source
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia +7 more
wiley +1 more source
Evaluation of a Novel Listeria Enrichment Broth Combined with a Real-Time PCR Diagnostics Assay for the Specific Detection of Listeria Monocytogenes in RTE Pork Products [PDF]
Anonymous +21 more
core +1 more source
Periodontitis treatment and microbiome in a patient with FAM20A mutation: Case study of 1.5 years
Abstract Background Enamel‐renal‐gingival syndrome (ERGS) is an autosomal recessive disorder caused by mutations in the FAMily with sequence similarity 20A (FAM20A) gene, and is characterized by amelogenesis imperfecta, delayed or failed tooth eruption, and periodontitis.
John Rong Hao Tay +2 more
wiley +1 more source
Refining a low-cost dermatophyte sampling method to enhance culture purity in resource-limited clinical settings in Bangladesh. [PDF]
Hasan M +7 more
europepmc +1 more source
Enrichment, isolation and some properties of methane-utilizing bacteria.
R. Whittenbury +2 more
semanticscholar +1 more source

