Integrating non‐chromatographic operations, such as low pH viral inactivation and a depth filtration mimic, into an automated micro‐scale platform enhances productivity while reducing resource and time requirements. By facilitating rapid, data‐rich experimentation, the platform offers a powerful and efficient tool for assessing and optimizing ...
Paras Sharma +4 more
wiley +1 more source
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Isolation of a <i>Gordonia rubripertincta</i> bacteriophage, BluerMoon, from topsoil in Lubbock, Texas. [PDF]
Miller LN +19 more
europepmc +1 more source
Dynamics of natural killer cell function upon recurrent stimulation
Weekly stimulation of peripheral blood‐derived NK cells with engineered K562 feeder cells resulted in two distinct growth phases, shifting around Day 21–26. Cells in growth phase 2 exhibited slower growth, reduced metabolic activity, and lower cytotoxicity than cells from growth phase 1, which has implications for NK cell biomanufacturing.
Jennifer One +4 more
wiley +1 more source
Correction: A new selective culture medium for isolation of <i>Burkholderia cepacia</i> complex in pharmaceutical industry. [PDF]
Yu M +7 more
europepmc +1 more source
Periodontitis treatment and microbiome in a patient with FAM20A mutation: Case study of 1.5 years
Abstract Background Enamel‐renal‐gingival syndrome (ERGS) is an autosomal recessive disorder caused by mutations in the FAMily with sequence similarity 20A (FAM20A) gene, and is characterized by amelogenesis imperfecta, delayed or failed tooth eruption, and periodontitis.
John Rong Hao Tay +2 more
wiley +1 more source
An interactive dashboard for global reports on the Ralstonia solanacearum species complex. [PDF]
Elmgreen VN +5 more
europepmc +1 more source
Following the Danube Flow. The first total syntheses of highly brominated rubrolides T, U, and U‐analog, together with the efficient and divergent syntheses of rubrolides A, D, and P. The dibrominated key intermediate, obtained via a Suzuki‐Miyaura cross‐coupling reaction, is the centerpiece of the synthesis.
Jasmin Janneschütz +7 more
wiley +1 more source
Draft genome sequence of <i>Nocardia</i> anissiorum JW2 isolated from soil with high antimicrobial resistance to antimicrobial compounds secreted by <i>Streptomyces spp</i>. [PDF]
Anissi J.
europepmc +1 more source

