Results 41 to 50 of about 323,080 (268)

Isolation of Helicobacter pylori from dental plaque: A microbiological study

Journal of Indian Society of Periodontology, 2008
The aim of our study was to isolate H. pylori from dental plaque in gastric and duodenal ulcer patients and compare it with dental plaque of healthy subjects.Fifty patients in the age range of 25-50 years who were endoscopically proven cases of duodenal and gastric ulcer were chosen. H.
Sudhakar, Uma, Anusuya, C. N., Ramakrishnan, T., Vijayalakshmi, R.   +3 more
openaire   +2 more sources

Swelling‐Programmed Topographical Guidance for Dynamic Spheroid Self‐Assembly via a Mechanochemical Hydrogel Niche

Advanced Functional Materials, EarlyView.
A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam, Seon Yeong Chae, Young Woo Kwon, Hye Jin Heo, Hye Rin Lee, Ji Hyeok Lee, Dong‐Wook Han, Yun Hak Kim, Suck Won Hong   +8 more
wiley   +1 more source

Analyzing human CD4+ T cells activated in response to macrophages infected with Mycobacterium tuberculosis

STAR Protocols
Summary: M1- and M2-like macrophages infected with Mycobacterium tuberculosis (Mtb) have been found to differ in their capacity to elicit memory CD4+ T cell activation. Here, we present a protocol to quantify and isolate the subset of human memory CD4+ T 
Daniel P. Gail, Vinicius G. Suzart, Stephen M. Carpenter   +2 more
doaj   +1 more source

Rhodotorula spp. isolated from blood cultures: clinical and microbiological aspects [PDF]

Medical Mycology, 2008
The emergence of less common fungal pathogens has been increasingly reported in the last decade. We describe 25 cases of Rhodotorula spp. isolated from blood cultures at a large Brazilian tertiary teaching hospital from 1996-2004. We also investigated the in vitro activity of four antifungal drugs, using a standardized method.
Gisele M Duboc, De Almeida, Silvia Figueiredo, Costa, Marcia, Melhem, Adriana L, Motta, Maria Walderez, Szeszs, Fumiko, Miyashita, Ligia C, Pierrotti, Flávia, Rossi, Marcelo N, Burattini   +8 more
openaire   +2 more sources

Characterization of an Injectable Poly(vinyl alcohol)‐gelatin Hydrogel for Growth Factor Delivery in an Orthopedic Application

Advanced Healthcare Materials, EarlyView.
This work introduces photo‐crosslinkable tyraminated poly(vinyl alcohol)‐gelatin (PVA‐GT) hydrogels as tunable injectable platforms for tissue engineering and growth factor delivery applications. This schematic illustrates the two developed hydrogel formulations and the experimental workflow used to evaluate their physico‐chemical properties in vitro ...
Alessia Longoni, Gretel S. Major, Sam Arnold, Samuel Tomkins, Eugenia Spessot, Scott Loeffler, Kieran Lau, Richard Tan, Jessica Harte, Roslyn Kemp, Tess Reynolds, Jelena Rnjak‐Kovacina, Gary J. Hooper, Tim B. F. Woodfield, David C. Kieser, Khoon S. Lim   +15 more
wiley   +1 more source

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

STAR Protocols
Summary: A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids.
Miner Deng, Yanting Wei, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo   +5 more
doaj   +1 more source

Co-existence of Network Architectures Supporting the Human Gut Microbiome

iScience, 2019
Summary: Microbial organisms of the human gut microbiome do not exist in isolation but form complex and diverse interactions to maintain health and reduce risk of disease development.
Caitlin V. Hall, Anton Lord, Richard Betzel, Martha Zakrzewski, Lisa A. Simms, Andrew Zalesky, Graham Radford-Smith, Luca Cocchi   +7 more
doaj   +1 more source

Isolated Discitis in Melioidosis: An Unknown Presentation and a Microbiological Challenge

Journal of Neurosciences in Rural Practice, 2021
Abstract Burkholderia pseudomallei causes Whitmore’s disease or melioidosis which is endemic in many South Asian countries including India. This gram-negative bacterium is frequently found in the moist soil and agricultural workers get infected most commonly.
Varma, Anoop, Mathew, Jacob Eapen, Gopalakrishnan, Chittur Viswanathan, Wilson, Arun   +3 more
openaire   +2 more sources

Enhanced Human Antigen‐Specific B Cell Responses Using In Vitro 3D Tonsil Cultures Containing Stromal Cells

Advanced Healthcare Materials, EarlyView.
We developed a fully human 3D tonsil cell culture system incorporating supportive stromal cells that better sustains and activates immune cells than conventional methods. The model generates stronger, more targeted antibody responses to viral antigens and vaccines, providing a physiologically relevant and entirely human platform for studying immune ...
Maaike V. J. Braham, Marlon de Gast, Liubov Babii, Sabine Kruijer, Theo M. Bestebroer, Mathilde Richard, Mathieu Claireaux, S. Marieke van Ham, Jelle de Wit, Cécile A. C. M. van Els, Anja ten Brinke, Marit J. van Gils   +11 more
wiley   +1 more source

Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses

STAR Protocols
Summary: Air-liquid interface (ALI) culture can differentiate airway epithelial cells to recapitulate the respiratory tract in vitro. Here, we present a protocol for isolating and culturing nasal epithelial cells from turbinate tissues for severe acute ...
Victor H.K. Lam, Aleena Ghafoor, Yazan Khan, Shirley Constable, Lane B. Buchanan, David Zuanazzi, Reeya Parmar, Zeynep G. Tepe, Leigh J Sowerby, Cindy M. Liu, Ryan M. Troyer, Jessica L. Prodger   +11 more
doaj   +1 more source