Results 101 to 110 of about 2,869 (126)
Binding mode of isoxazolyl penicillins to a Class-A β -lactamase at ambient conditions
Gore G +4 more
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CCLX.—Studies in chemical crystallography. Part I. Co-ordination, isomorphism, and valency
Journal of the Chemical Society Transactions, 1912exaly +2 more sources
Rare earths as isomorphous calcium replacements for protein crystallography
Biochemical and Biophysical Research Communications, 1972Abstract Replacement of calcium in thermolysin by lanthanide ions has been found to provide a useful isomorphous derivative for the X-ray analysis of the protein. The substitution can be achieved simply by diffusion of the heavy metal ions into the native protein crystals and, as measured by crystallographic residuals, causes little disruption of the
P M, Colman, L H, Weaver, B W, Matthews
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Ta6Br14 is a useful cluster compound for isomorphous replacement in protein crystallography
Acta Crystallographica Section D Biological Crystallography, 1994The metal cluster Ta(6)Br(14) has been used to prepare heavy-metal derivatives of two large proteins, ribulose-1,5-bisphosphate carboxylase/oxygenase and transketolase. In both cases, this cluster compound produced a single-site derivative for which a difference Patterson map, calculated to 5.5 A resolution, could be solved straightforwardly.
G, Schneider, Y, Lindqvist
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Isomorphous replacement with optimized anomalous scattering applied to protein crystallography
Acta Crystallographica Section A Foundations of Crystallography, 1990Isomorphous replacement is an essential technique for the de novo solution of macromolecular crystal structures. The use of the anomalous-dispersion effect of the heavy atom in the derivative leads to the acronyms SIRAS or MIRAS (single or multiple isomorphous replacement with anomalous scattering) as the term for the phase determination method ...
P. J. Baker +5 more
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A new isomorph of ferrous chloride tetrahydrate: A 57Fe Mössbauer and X-ray crystallography study
Journal of Physics and Chemistry of Solids, 2010Abstract This paper outlines the discovery of a newly characterised isomorph of ferrous chloride tetrahydrate, Fe(H2O)6·FeCl4(H2O)2, which was initially identified by X-ray crystallography and confirmed by Mossbauer spectroscopy. The X-ray analysis identified the space group as P21/c with essentially the same unit cell dimensions as the well-known ...
Bull, James N. +3 more
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Journal of Applied Crystallography, 2009
A rapid and simple X-ray fluorescence-based method is reported for characterizing heavy atom derivatives of proteins for protein crystallography using multiple isomorphous replacement (MIR). MIR is a widely used technique for solving protein crystallographic structures which requires that a `heavy atom' be incorporated into the protein to provide a ...
Leslie A. Colip +8 more
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A rapid and simple X-ray fluorescence-based method is reported for characterizing heavy atom derivatives of proteins for protein crystallography using multiple isomorphous replacement (MIR). MIR is a widely used technique for solving protein crystallographic structures which requires that a `heavy atom' be incorporated into the protein to provide a ...
Leslie A. Colip +8 more
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"Protein Engineering, Design and Selection", 1987
Five different cysteine-containing mutants of the lysozyme from bacteriophage T4 were used to explore the feasibility of using site-directed mutagenesis to generate isomorphous heavy-atom derivatives for protein crystallography. Cysteines 54 and 97, present in wild-type lysozyme, can be readily reacted with mercuric ion to produce an excellent ...
D P, Sun +4 more
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Five different cysteine-containing mutants of the lysozyme from bacteriophage T4 were used to explore the feasibility of using site-directed mutagenesis to generate isomorphous heavy-atom derivatives for protein crystallography. Cysteines 54 and 97, present in wild-type lysozyme, can be readily reacted with mercuric ion to produce an excellent ...
D P, Sun +4 more
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Acta Crystallographica Section A Foundations of Crystallography, 1983
A correct calculation of the scale factor between the diffraction data of isomorphous crystals is shown on the basis of a statistics criterion. This scale factor requires no additional refinement, improves the signal-to-noise ratio of difference Patterson maps and Wilson's statistics and allows an estimation of the degree of non-isomorphism. The method
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A correct calculation of the scale factor between the diffraction data of isomorphous crystals is shown on the basis of a statistics criterion. This scale factor requires no additional refinement, improves the signal-to-noise ratio of difference Patterson maps and Wilson's statistics and allows an estimation of the degree of non-isomorphism. The method
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The application of MULTAN to the analysis of isomorphous derivatives in protein crystallography
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry, 1978openaire +1 more source