Real‐time NMR spectroscopy is used to study JNK1‐dependent phosphorylation of the intrinsically disordered substrate JIP1, revealing eleven phosphosites with distinct phosphorylation efficiencies. The site‐specific phosphorylation rates are shown to be determined by both the sequence position of the phosphosites relative to the two kinase docking site ...
Thibault Orand+7 more
wiley +1 more source
Incorporation of Isotopically Labeled Precursors into the Pyridine Nucleotide Coenzymes
Victor Stollar, Nathan O. Kaplan
openalex +1 more source
DeepSecMS Advances DIA‐Based Selenoproteome Profiling Through Cys‐to‐Sec Proxy Training
DeepSecMS, a deep learning platform leveraging a Cys‐to‐Sec proxy training strategy, enhances the identification of known selenoproteins and holds promise for discovering novel candidates. This proxy approach overcomes limitations associated with the rarity of known selenoproteins and demonstrates broad applicability in studying other rare protein ...
Chenfang Si+5 more
wiley +1 more source
Turnover and replication analysis by isotope labeling (TRAIL) reveals the influence of tissue context on protein and organelle lifetimes. [PDF]
Hasper J+4 more
europepmc +1 more source
Evidence for light-induced 13-cis , 14-s-cis isomerization in bacteriorhodopsin obtained by FTIR difference spectroscopy using isotopically labelled retinals [PDF]
Klaus Gerwert, Friedrich Siebert
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The complex centered on Ghd7 binds to numerous carbon and nitrogen metabolism genes on the genome through the corresponding motifs and transactivates the transcriptional expression of these carbon and nitrogen metabolism genes. With the assistance of OsNAC42 and other unknown Ghd7‐interacting proteins, this complex can regulate different metabolic ...
Guangming Lou+23 more
wiley +1 more source
Isotope-labeling in situ derivatization and HS-SPME arrow GC-MS/MS for simultaneous determination of fatty acids and fatty acid methyl esters in aqueous matrices. [PDF]
Tintrop LK+6 more
europepmc +1 more source
Cu5%‐MoS2 with dual Cu and Mo active sites enables efficient photothermal synergistic catalysis of the CO2–H2O system for ethanol production. The catalyst achieves a high ethanol yield of 3.1 mmol·g−1·h−1 with 88.35% selectivity, showcasing exceptional performance and selectivity under mild conditions. Abstract The catalytic conversion of CO2 into high‐
Yingao Luo+6 more
wiley +1 more source
Advances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis. [PDF]
Beller NC, Hummon AB.
europepmc +1 more source