Results 301 to 310 of about 100,704 (351)
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Analytical Biochemistry, 1981
Abstract Thiamine of high specific radioactivity has been prepared by catalytic incorporation of tritium using 3H2O and platinum. Purification of a crude commercially available product is described and the radiolabeling pattern is determined by NMR analysis of deuterated thiamine.
D R, Doerge, T, Sakurai, L L, Ingraham
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Abstract Thiamine of high specific radioactivity has been prepared by catalytic incorporation of tritium using 3H2O and platinum. Purification of a crude commercially available product is described and the radiolabeling pattern is determined by NMR analysis of deuterated thiamine.
D R, Doerge, T, Sakurai, L L, Ingraham
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2012
A great variety of technologies using stable isotope labeling in combination with mass spectrometry have been described being tools to identify and relatively quantify proteins within complex mixtures. Here, we present a method, termed isotope-coded protein label (ICPL), which is capable of high-throughput quantitative proteome profiling on a global ...
Kellermann, J., Lottspeich, F.
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A great variety of technologies using stable isotope labeling in combination with mass spectrometry have been described being tools to identify and relatively quantify proteins within complex mixtures. Here, we present a method, termed isotope-coded protein label (ICPL), which is capable of high-throughput quantitative proteome profiling on a global ...
Kellermann, J., Lottspeich, F.
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Glutathione specifically labeled with isotopes
Analytical Biochemistry, 1985A procedure for synthesis of glutathione selectivity labeled with isotopes is described. A strain of Escherichia coli enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase by recombinant DNA techniques is immobilized in a carrageenan matrix and treated with toluene to render the cells more permeable to the substrates ...
K, Murata +3 more
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SAIL – stereo-array isotope labeling
Quarterly Reviews of Biophysics, 2009AbstractOptimal stereospecific and regiospecific labeling of proteins with stable isotopes enhances the nuclear magnetic resonance (NMR) method for the determination of the three-dimensional protein structures in solution. Stereo-array isotope labeling (SAIL) offers sharpened lines, spectral simplification without loss of information and the ability to
Masatsune, Kainosho, Peter, Güntert
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Isotope Labeling in Insect Cells
2012Target proteins in contemporary NMR are becoming increasingly complex. The interest lies on membrane proteins, multi-domain proteins, secreted proteins with secondary modifications etc., which can often only be expressed in eukaryotic expression hosts. Although E. coli is the organism of choice for expression of proteins in isotope labeled form for NMR
Alvar D, Gossert, Wolfgang, Jahnke
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Stable Isotope Labeling in Mammals (SILAM)
Cold Spring Harbor Protocols, 2008INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., 2H, 13C, 15N, or 18O).
Daniel B, McClatchy, John R, Yates
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Labeling Monosaccharides With Stable Isotopes
2015Chemical and chemi-enzymic methods are discussed for the preparation of monosaccharide isotopomers that are singly and multiply labeled with (13)C, (2)H, (17/18)O, and (15)N isotopes. The discussion focuses primarily on chemical methods to incorporate stable isotopes into monosaccharides and not on methods to assemble labeled monosaccharides into more ...
Wenhui, Zhang +2 more
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ICPL—Isotope-Coded Protein Label
2008Stable isotope labeling in combination with mass spectrometry has emerged as a powerful tool to identify and relatively quantify thousands of proteins within complex protein mixtures. Here we describe a method, termed isotope-coded protein label (ICPL), which is capable of high-throughput quantitative proteome profiling on a global scale.
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Production of Isotopically Labeled KRAS4b
Isotopically labelled proteins are important reagents in structural biology as well as in targeted drug development. The field continues to advance with complex multi-isotope labeling. We have combined our experience in high-level soluble KRAS4b expression with protocols for isotope incorporation, to achieve reliable and efficient approaches for ...Troy, Taylor, William, Gillette
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Unsupervised Identification of Isotope-Labeled Peptides
Analytical Chemistry, 2016In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon substrates and intracellular metabolism determine the distribution of isotopes within amino acids.
Joshua E, Goldford, Igor G L, Libourel
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