Novel ITS1 Fungal Primers for Characterization of the Mycobiome [PDF]
mSphere, 2017 The mycobiome constitutes all the fungal organisms within an environment or biological niche. The fungi are eukaryotes, are extremely heterogeneous, and include yeasts and molds that colonize humans as part of the microbiome.
Mykhaylo Usyk +4 more
semanticscholar +4 more sources
Experimental quantification of pollen with DNA metabarcoding using ITS1 and trnL [PDF]
Scientific Reports, 2020 Although the use of metabarcoding to identify taxa in DNA mixtures is widely approved, its reliability in quantifying taxon abundance is still the subject of debate.
Sandra Baksay +5 more
semanticscholar +5 more sources
Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing
Chinese MedicineBackground Fritillariae Cirrhosae Bulbus is an antitussive and expectorant Chinese medicinal material derived from the dried bulbs of six Fritillaria species.
Hoi-Yan Wu +8 more
doaj +3 more sources
Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact [PDF]
BioMed Research International, 2014 The utility of various regions of the ribosomal repeat unit for phylogenetic analysis was examined in 16 species representing four families, nine genera, and two orders of the subclass Copepoda (Crustacea).
M. Zagoskin +3 more
semanticscholar +3 more sources
Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing [PDF]
Journal of Cell Biology, 2013 Human ribosomal RNA processing is initiated by endonuclease cleavage and followed by 3′ to 5′ exonucleolytic processing by RRP6 and the exosome.
K. Sloan +5 more
semanticscholar +5 more sources
bioRxiv, 2021 Identification and analysis of fungal communities commonly rely on internal transcribed spacer (ITS)-based amplicon sequencing. Currently, there is no gold standard to infer and classify fungal constituents, in part since methodologies have been adapted ...
T. Rolling +4 more
semanticscholar +1 more source
kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite [PDF]
Journal of Kerman University of Medical Sciences, 2019 Background: Cutaneous Leishmaniasis, is endemically observed in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention.
Hadi Mirahmadi +5 more
doaj +1 more source
Utilizing ITS1 and ITS2 to study environmental fungal diversity using pyrosequencing. [PDF]
FEMS Microbiology Ecology, 2013 The shorter reads generated by high-throughput sequencing has led to a focus on either the ITS1 or the ITS2 sublocus in fungal diversity analyses. Our study aimed to determine how making this choice would influence the datasets obtained and our vision of environmental fungal diversity.
C. Monard, S. Gantner, J. Stenlid
semanticscholar +5 more sources
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates [PDF]
, 2013 Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B.
Grim, J Norman +9 more
core +8 more sources
Limitations of Common Molecular Markers in Fungal Biodiversity Analysis and the Benefits of Their Synergistic Use. [PDF]
Mol Ecol ResourABSTRACT High‐throughput sequencing of the Internal Transcribed Spacer (ITS) regions is the primary method for estimating fungal diversity from environmental DNA. However, reliance solely on ITS markers is complicated by its high variability in sequence length and the presence of multiple variants within a single genome, which can bias diversity ...
Shapkin V +11 more
europepmc +2 more sources