Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing
Background Fritillariae Cirrhosae Bulbus is an antitussive and expectorant Chinese medicinal material derived from the dried bulbs of six Fritillaria species.
Hoi-Yan Wu +8 more
doaj +3 more sources
Novel ITS1 Fungal Primers for Characterization of the Mycobiome [PDF]
The mycobiome constitutes all the fungal organisms within an environment or biological niche. The fungi are eukaryotes, are extremely heterogeneous, and include yeasts and molds that colonize humans as part of the microbiome. In addition, fungi can also infect humans and cause disease.
Mykhaylo Usyk +4 more
openaire +3 more sources
Prediction of potential barcoding sites on ITS1 by wavelet transform [PDF]
For sustainable development, biodiversity conservation and life-quality improvement must be simultaneously considered. Molecular techniques have greatly impacted biotechnology. These methods have, in particular, improved the capability to investigate the fine differences among organisms and, as a consequence, to better investigate the effects on ...
MAGGI, NORBERT +2 more
openaire +3 more sources
Experimental quantification of pollen with DNA metabarcoding using ITS1 and trnL [PDF]
AbstractAlthough the use of metabarcoding to identify taxa in DNA mixtures is widely approved, its reliability in quantifying taxon abundance is still the subject of debate. In this study we investigated the relationships between the amount of pollen grains in mock solutions and the abundance of high-throughput sequence reads and how the relationship ...
Baksay, Sandra +5 more
openaire +4 more sources
Slx9p facilitates efficient ITS1 processing of pre-rRNA in Saccharomyces cerevisiae [PDF]
Slx9p (Ygr081cp) is a nonessential yeast protein previously linked genetically with the DNA helicase Sgs1p. Here we report that Slx9p is involved in ribosome biogenesis in the yeast Saccharomyces cerevisiae. Deletion of SLX9 results in a mild growth defect and a reduction in the level of 18S rRNA. Co-immunoprecipitation experiments showed that Slx9p is
Bax, R., Raue, H.A., Vos, J.C.
openaire +2 more sources
Utilizing ITS1 and ITS2 to study environmental fungal diversity using pyrosequencing [PDF]
The shorter reads generated by high-throughput sequencing has led to a focus on either the ITS1 or the ITS2 sublocus in fungal diversity analyses. Our study aimed to determine how making this choice would influence the datasets obtained and our vision of environmental fungal diversity.
Monard, Cécile +2 more
openaire +4 more sources
Efficiency of ITS1-5.8S-ITS2 region in identifying Cordyceps species [PDF]
Cordyceps genus is a well-known traditional medicine worldwide. It contains abundant physiological active compounds that were demonstrated to perform benefit in reducing progression of cancer as well as protecting human health. Accurately classifying species in this genus is essential in order to prevent commercial counterfeit medicines.
Le Thi Thu Hien, Ha Hong Hanh
openaire +1 more source
The impact of one-decade ecological disturbance on genetic changes : a study on the brine shrimp Artemia urmiana from Urmia Lake, Iran [PDF]
Urmia Lake, the largest natural habitat of the brine shrimp Artemia urmiana, has progressively desiccated over the last two decades, resulting in a loss of 80% of its surface area and producing thousands of hectares of arid salty land.
Asem, Alireza +3 more
core +1 more source
Intraspecific Variation and Phylogenetic Relationships Are Revealed by ITS1 Secondary Structure Analysis and Single-Nucleotide Polymorphism in Ganoderma lucidum. [PDF]
Ganoderma lucidum is a typical polypore fungus used for traditional Chinese medical purposes. The taxonomic delimitation of Ganoderma lucidum is still debated.
Xiuqing Zhang +7 more
doaj +1 more source
Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal [PDF]
A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central ...
Castillo, P +4 more
core +1 more source

