PCR amplification and sequencing of ITS1 rDNA of Culicoides arakawae
Veterinary Parasitology, 2003The first internal transcribed spacer (ITS1) of nuclear ribosomal DNA from Culicoides arakawae was amplified by PCR, cloned and sequenced. The wDNAsis software was used to analyze the ITS1 sequences of C. arakawae and other nine species of Culicoides, which were obtained from GenBank and EMBL databases. For all species, the lengths of the ITS1 were 316-
G Q, Li, Y L, Hu, S, Kanu, X Q, Zhu
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Heterogeneity of the internal transcribed spacer 1 (ITS1) inTulipa (Liliaceae)
Plant Systematics and Evolution, 2000Heterogeneity of the internal transcribed spacer ITS1 of the rDNA within individuals ofTulipa gesneriana L.,T. kaufmanniana Regel, and their interspecific hybrids was analyzed by PCRRFLP, using the polymorphic restriction enzymesRsaI andHinfI, and by nucleotide sequence analysis.
Booy, G., van der Schoot, J., Vosman, B.
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Regulatory motifs are present in the ITS1 of some flatworm species
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 2003AbstractParticular sequence motifs can act as transcription regulators. Because the total regulatory effects of such motifs can be related to their abundance, their presence might be expected at locations within the genome where sequences are repeated.
van Herwerden, Lynne +2 more
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Conserved motifs in the primary and secondary ITS1 structures of bryophytes
Molecular Biology, 2015A study of the ITS1 nucleotide sequences of 1000 moss species of 62 families, 11 liverwort species from five orders, and one hornwort Anthoceros agrestis identified five highly conserved motifs (CM1-CM5), which are presumably involved in pre-rRNA processing.
I A, Milyutina, M S, Ignatov
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The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomes
Parasitology Research, 2004There are 11 different pathogenic trypanosomes in trypanosomiasis endemic regions of Africa. Their detection and characterisation by molecular methods relies on species-specific primers; consequently several PCR tests have to be made on each sample. Primers ITS1 CF and ITS1 BR, previously designed to amplify the internal transcribed spacer (ITS1) of ...
Njiru, Z.K. +6 more
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Evolving lineages of Symbiodinium-like dinoflagellates based on ITS1 rDNA
Molecular Phylogenetics and Evolution, 2003Symbiodinium-like dinoflagellates have been shown to be a diverse group of endosymbionts that associate mutualistically with many kinds of coral reef dwellers, including cnidarians, molluscs, and protists. A high number of genetically ITS types of symbionts have been reported to date.
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Size and sequence heterogeneity in the ITS1 of Xylaria hypoxylon isolates
Mycological Research, 2004During a survey of 375 strains of the Xylariales, one isolate (F127076) was observed to have an ITS1 size of 833 bp. This size exceeds the average ITS1 size in the Xylariales (mean = 209 +/- 57 bp). Comparison of the DNA sequence with GenBank and with a proprietary DNA database revealed low homology with Xylaria hypoxylon ATCC 42768, and with one ...
Gonzalo, Platas +2 more
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Ascaridia galli isolates with ITS1-5.8rRNA-ITS2 fragment homologous to Ascaridia columbae
Acta Parasitologica, 2018Abstract Ascaridia (A.) galli is one of the most commonly occurring nematodes in poultry worldwide, often in hens and broiler chickens. The infection with Ascaridia galli in free-range chickens was even 70%. There is not much information about A. galli genetic features. The present study was conducted to assess the genetic diversity of A.
Joanna, Urbanowicz +2 more
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Detection of filarial parasites in domestic cats by PCR-RFLP of ITS1
Veterinary Parasitology, 2006Lymphatic filariasis has been targeted by the World Health Organization (WHO) to be eliminated by the year 2020. In addition to chemotherapy and vector control, the control of reservoir hosts is necessary for the control program to succeed. Malayan filariasis, caused by Brugia malayi, is endemic in the South of Thailand where domestic cats serve as the
Surang, Nuchprayoon +5 more
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Phylogenetic relationships among six species of Epistylis inferred from 18S-ITS1 sequences
Science in China Series C, 2002Phylogenetic relationships among six species of Epistylis (i. e. E. plicatilis, E. urceolata, E. chrysemydis, E. hentscheli, E. wenrichi, and E. galea) were investigated using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA). Amplified rDNA fragment sequences consisted of 215 or 217 bases of the flanking 18S and
Wei, Miao +3 more
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