Molecular Oncology, EarlyView.This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan +8 more
wiley +1 more source
Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer
Molecular Oncology, EarlyView.New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau +9 more
wiley +1 more source
ENaC and Tight Junction Proteins: Coordinated Regulation of Alveolar Fluid Homeostasis in ALI/ARDS. [PDF]
Pharmaceuticals (Basel)Li S, Wang J, Yin Z, Xin S, Nie H, Yu T.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A simple and cost-effective denaturation-reannealing multiplex PCR (drmPCR) method for discriminating late-feathering genotypes in male Korean indigenous breed chickens. [PDF]
Poult SciLee ES +7 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Imaging of Craniovertebral Junction
, 2011 This article reviews the embryology, anatomy, congenital anomaly, acquired disorders, degenerative diseases, neoplasms, and inflammatory and infectious disorders in the craniovertebral junction.
陳雅芳;廖漢文 +1 more
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Ceramic Embedded Junction Thermocouple for In Situ Transient Temperature Measurement in Confined Extreme Thermal Environments. [PDF]
Micromachines (Basel)E M +6 more
europepmc +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Negative differential resistance in molecular junctions. [PDF]
RSC AdvKrishnapriya R +9 more
europepmc +1 more source